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Glycobiology Advance Access published online on November 26, 2002
Glycobiology, doi:10.1093/glycob/cwg016
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Submitted on July 9, 2002
Revised on September 23, 2002
Accepted on September 23, 2002

© 2002 Oxford University Press

ORIGINAL ARTICLES

The effects of ethanol on the glycosylation of human transferrin

C. Flahaut 1, J.C. Michalski 1, T. Danel 2, M.H. Humbert 3, A. Klein 4*

1 UMR 8576 du CNRS, Laboratoire de Glycobiologie Structurale et Fonctionnelle, et IFR 118 USTL, 59655, Villeneuve d'Ascq, France
2 Clinique de la Charité, CHRU de Lille, 59045, Lille, France
3 Laboratoire de Biochimie et de Biologie Moléculaire, Hôpital Calmette Bld du Professeur Jules Leclerc, Lille 59037 Cedex, France
4 Laboratoire de Biochimie et de Biologie Moléculaire, Hôpital Calmette Bld du Professeur Jules Leclerc, Lille 59037 Cedex, France.

* To whom correspondence should be addressed. E-mail: a-klein{at}chru-lille.fr.

Abstract

Appearance of a hyposialylated transferrin fraction in the plasma during chronic alcohol exposure is a well known phenomenon and it represents the best available marker of chronic alcohol consumption. The mechanisms of its appearance are still not well understood and are extremely complex, involving biosynthesis and catabolism alterations, although the only structural abnormality described corresponds to the loss of an entire glycan chain. In this study, we have analysed and compared the oligosaccharides present on the different isoforms of purified transferrin isolated from control and patients with severe alcohol abuse by fluorescent carbohydrate electrophoresis and Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI-MS). Our data indicate that the major modification observed is the loss of an entire oligosaccharide chain and we demonstrate also that there is a modification of terminal sialylation. CDT (carbohydrate deficient transferrin) is the result of multiple alterations of glycosylation. These results give a partial explanation to the poor sensitivity of the measurement of CDT and its controversial use as a marker of chronic alcohol consumption.


Key words : Alcohol / carbohydrate deficient transferrin (CDT) / hyposialylation
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