Glycobiology, Vol 9, 907-914, Copyright © 1999 by Oxford University Press
NW Lo and JT Lau
A single gene, SIAT1, encodes ST6Gal I, the sialyltransferase that mediates
transfer of alpha2,6-linked sialic acids to Galbeta1, 4GlcNAc termini of
N-linked glycoproteins. In vivo, multiple SIAT1 mRNA forms, differing only
in the 5'-untranslated region, are expressed in a tissue- specific manner.
This mRNA heterogeneity has been attributed, at least in part, to
transcription from a number of physically distinct promoter regions. In
mature B-lymphocytes, SIAT1 transcription initiates at P2, a regulatory
region known to function only in B-lineage cells. Bacterial chloramphenicol
acetyltransferase (CAT) under the control of the P2 region encompassing 415
bp 5'- and 125 bp 3' of the transcriptional initiation site is efficiently
expressed in Louckes, a mature B-lymphoblastoid cell line. In contrast, CAT
expression in Reh, a T-null/B-null precursor line, and in HepG2, a hepatoma
line, are 14- fold and >25-fold less than in Louckes, respectively. The
data is consistent with the presence of cis -acting regulatory elements
residing both 5' and 3' of the P2 transcriptional initiation site. At least
370 bp of 5'-flanking sequence, coinciding with the inclusion of AP2 and
NF-kappaB sites, is necessary for high level expression in Louckes. Exon
sequences 3' of the transcription start site are also important for
expression. A segment from(+)32 to(+)125 (position(+)1 is transcription
start site) is capable of exerting promoter-like activity in Louckes, but
not in Reh or HepG2. CAT expression by P2 is negligible in Reh cells.
However, enhanced CAT activity is not accompanied by elevated mRNA levels.
This observation is consistent with the relief of translational restraints
imposed by the(+)32 to(+)125 region. Together, the data demonstrate that
efficient and cell-specific transcription regulation in mature B
lymphocytes is contained in a 495 bp P2 segment that is comprised of 370 bp
of 5'-flanking region and 125 bp of transcribed region of Exon X.
ORIGINAL ARTICLES
Transcription of the beta-galactoside alpha2,6-sialyltransferase gene (SIAT1) in B-lymphocytes: cell type-specific expression correlates with presence of the divergent 5'-untranslated sequence
Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA.
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