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Glycobiology, 1999, Vol. 9, No. 12 1337-1345
© 1999 Oxford University Press

Glycosylation alterations ofcells in late phase apoptosis from colon carcinomas

Evgenia Rapoporta and JacquesLe Pendub

INSERM U419, Institut de Biologie, 9 Quai Moncousu, 44035,Nantes, Cedex, France

Comparisons of carbohydrate profiles between controland apoptotic colon carcinoma cells were performed by flow cytometryusing a set of lectins and anti-carbohydrate antibodies. The sixcell lines analyzed presented distinct carbohydrate profiles beforeinduction of apoptosis. PHA-L and MAA binding decreased after inductionof apoptosis by UV-treatment. In contrast an increase of PNA bindingwas observed after induction of apoptosis, except on SW-48 cellsfor which a decrease occurred. A decrease of SNA binding was observedafter induction of apoptosis from strongly positive control celllines, whereas it increased on weakly positive ones. All the bloodgroup related antigens A, H, Lewis a, Lewis x, Lewis b, and Lewisy, had their expression strongly diminished on apoptotic cells.These changes occurred irrespective of the mode of apoptosis inductionsince similar results were obtained after UV, TNF{alpha},or anti-Fas treatment. Fucosyltransferases activities were alsodecreased after apoptosis induction, except for {alpha}1,3fucosyltransferasein anti-Fas treated HT-29 cells, where it was strongly augmented.This could be attributed to the IFN{gamma} preteatmentrequired to induce Fas expression on these cells. Fucosidase activitydecreased after induction of apoptosis suggesting that it was notresponsible for the loss of fucosylated structures. In the rat PROcell line, H blood group antigens are mainly carried by a high molecularweight variant of CD44. It could be shown that the loss of H antigenafter induction of apoptosis correlated with a loss of the carrierglycoprotein.

a Presentaddress: Shemyakin Institute of Bioorganic Chemistry, Moscow, Russia

b To whom correspondenceshould be addressed


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