Glycobiology, Vol 9, 73-81, Copyright © 1999 by Oxford University Press
S Olofsson, A Bolmstedt, M Biller, K Mardberg, J Leckner, BG Malmstrom, E Trybala and T Bergstrom
A monoclonal antibody, B1C1, binding to an epitope of antigenic site II of
the herpes simplex virus type 1 (HSV-1) glycoprotein gC-1, is a potent
inhibitor of two important biological functions of gC-1: its binding to
cell surface heparan sulfate and its binding to the receptor for complement
factor C3b. Here, we have analyzed a B1C1-resistant HSV- 1 variant
(HSV-12762/B1C1B4.2), obtained after passage of wild type HSV- 1
(HSV-12762) in the presence of high concentrations of B1C1. The transport
of newly synthesized mutant gC-1 to the cell surface was comparable to that
of wild type glycoprotein, but no binding of surface- associated mutant
gC-1 to B1C1 was detected. However, mutant and wild type gC-1 bound equally
well to other site II Mabs. Attachment of wild type but not mutant virus
was inhibited by B1C1. Sequencing of the mutant gC-1 gene revealed only one
nucleotide change, resulting in replacement of Thr150 by an Ile, in turn
destroying an N-glycosylation site at Asn148. Loss of one complex type
N-linked glycan was confirmed by endoglycosidase digestion and subsequent
SDS-polyacrylamide gel electrophoresis. Circular dichroism analysis of
purified gC-1 from cells infected with mutant or wild type virus did not
reveal any difference in secondary structure between mutant and wild type
gC-1. It was not possible to obtain a B1C1-resistant phenotype by
nucleotide- directed mutagenesis of gC-1 where Asn148 was changed to a
glutamine. These data demonstrated that the threonine of the glycosylation
site and not the N-linked glycan in itself was essential for B1C1 binding
ORIGINAL ARTICLES
The role of a single N-linked glycosylation site for a functional epitope of herpes simplex virus type 1 envelope glycoprotein gC
Department of Clinical Virology, University of Goteborg, Guldhedsgatan 10 B, S-413 46 Goteborg, Sweden.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. R. Teuton and C. R. Brandt Sialic Acid on Herpes Simplex Virus Type 1 Envelope Glycoproteins Is Required for Efficient Infection of Cells J. Virol., April 15, 2007; 81(8): 3731 - 3739. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Mardberg, K. Nystrom, M. A. Tarp, E. Trybala, H. Clausen, T. Bergstrom, and S. Olofsson Basic amino acids as modulators of an O-linked glycosylation signal of the herpes simplex virus type 1 glycoprotein gC: functional roles in viral infectivity Glycobiology, July 1, 2004; 14(7): 571 - 581. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Mardberg, E. Trybala, J. C. Glorioso, and T. Bergstrom Mutational analysis of the major heparan sulfate-binding domain of herpes simplex virus type 1 glycoprotein C J. Gen. Virol., August 1, 2001; 82(8): 1941 - 1950. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Bolmstedt, B. R. O'Keefe, S. R. Shenoy, J. B. McMahon, and M. R. Boyd Cyanovirin-N Defines a New Class of Antiviral Agent Targeting N-Linked, High-Mannose Glycans in an Oligosaccharide-Specific Manner Mol. Pharmacol., April 16, 2001; 59(5): 949 - 954. [Abstract] [Full Text]
|
|
|
|
 |
|
 J.-A. Liljeqvist, B. Svennerholm, and T. Bergström Conservation of Type-Specific B-Cell Epitopes of Glycoprotein G in Clinical Herpes Simplex Virus Type 2 Isolates J. Clin. Microbiol., December 1, 2000; 38(12): 4517 - 4522. [Abstract] [Full Text]
|
|
|
|
|
|
M. Biller, K. Mardberg, H. Hassan, H. Clausen, A. Bolmstedt, T. Bergstrom, and S. Olofsson Early steps in O-linked glycosylation and clustered O-linked glycans of herpes simplex virus type 1 glycoprotein C: effects on glycoprotein properties Glycobiology, December 1, 2000; 10(12): 1259 - 1269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-A. Liljeqvist, B. Svennerholm, and T. Bergström Herpes Simplex Virus Type 2 Glycoprotein G-Negative Clinical Isolates Are Generated by Single Frameshift Mutations J. Virol., December 1, 1999; 73(12): 9796 - 9802. [Abstract] [Full Text]
|
|
|
|
|
|
J.-A. Liljeqvist, B. Svennerholm, and T. Bergström Typing of Clinical Herpes Simplex Virus Type 1 and Type 2 Isolates with Monoclonal Antibodies J. Clin. Microbiol., August 1, 1999; 37(8): 2717 - 2718. [Abstract] [Full Text]
|
|