Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (15)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Eckhardt, M.
Right arrow Articles by Gerardy-Schahn, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Eckhardt, M.
Right arrow Articles by Gerardy-Schahn, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Glycobiology, Vol 8, 1165-1172, Copyright © 1998 by Society for Glycobiology

ORIGINAL ARTICLES

Genomic organization of the murine polysialyltransferase gene ST8SiaIV (PST-1)

M Eckhardt and R Gerardy-Schahn
Institut fur Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Germany.

Polysialic acid (PSA) is an important regulator of cellular interactions. Two enzymes (ST8SiaII and ST8SiaIV) are capable of synthesizing PSA. In the present study, the gene encoding the murine ST8SiaIV (PST-1) has been isolated and characterized. In contrast to the ST8SiaII (STX) gene which contains six exons and spans about 80 kb, the ST8SiaIV gene comprises only five exons spanning over at least 55 kb. However, alignment of the two genes revealed that exon-intron boundaries of exons 2-5 of ST8SiaIV and exons 3-6 of ST8SiaII are located at identical sites. Differences are restricted to the 5'-region encoded by one exon in the case of ST8SiaIV, whereas the corresponding region of ST8SiaII is interrupted by a very long intron. 5'-RACE analysis of the ST8SiaIV transcript using mRNA from AtT20 cells identified two transcription start sites at positions -324 and -204 relative to the translation start codon. The promoter region of ST8SiaIV lacks TATA- and CAAT-like sequences and is enriched in G+C (60%). The promoter contains putative Sp1, AP-1, AP-2, and PEA3 binding sites, as well as a purine- and a pyrimidine-rich region. Luciferase reporter gene assays demonstrated that the region between nucleotides - 443 and -162 is sufficient to direct gene expression. The induction of luciferase activity was 30- and 10-fold in the PSA-positive AtT20 and CHO cells, but only 5- and 7-fold in the PSA-negative NIH-3T3 cells and in a PSA-negative subline of AtT20. Thus, although decreased in activity in PSA-negative cell lines, the basal promoter is not sufficient for the strong cell-type and tissue specific regulation of the ST8SiaIV gene, suggesting regulatory elements in the more upstream 5'-region.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Pharmacol.Home page
A. Lampen, P. A. Grimaldi, and H. Nau
Modulation of Peroxisome Proliferator-Activated Receptor {delta} Activity Affects Neural Cell Adhesion Molecule and Polysialyltransferase ST8SiaIV Induction by Teratogenic Valproic Acid Analogs in F9 Cell Differentiation
Mol. Pharmacol., July 1, 2005; 68(1): 193 - 203.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Angata, J. M. Long, O. Bukalo, W. Lee, A. Dityatev, A. Wynshaw-Boris, M. Schachner, M. Fukuda, and J. D. Marth
Sialyltransferase ST8Sia-II Assembles a Subset of Polysialic Acid That Directs Hippocampal Axonal Targeting and Promotes Fear Behavior
J. Biol. Chem., July 30, 2004; 279(31): 32603 - 32613.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Takashima, H.-k. Ishida, T. Inazu, T. Ando, H. Ishida, M. Kiso, S. Tsuji, and M. Tsujimoto
Molecular Cloning and Expression of a Sixth Type of alpha 2,8-Sialyltransferase (ST8Sia VI) That Sialylates O-Glycans
J. Biol. Chem., June 28, 2002; 277(27): 24030 - 24038.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
S. Hemmerich, J. K. Lee, S. Bhakta, A. Bistrup, N. R. Ruddle, and S. D. Rosen
Chromosomal localization and genomic organization for the galactose/ N-acetylgalactosamine/N-acetylglucosamine 6-O-sulfotransferase gene family
Glycobiology, January 1, 2001; 11(1): 75 - 87.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. Eckhardt, O. Bukalo, G. Chazal, L. Wang, C. Goridis, M. Schachner, R. Gerardy-Schahn, H. Cremer, and A. Dityatev
Mice Deficient in the Polysialyltransferase ST8SiaIV/PST-1 Allow Discrimination of the Roles of Neural Cell Adhesion Molecule Protein and Polysialic Acid in Neural Development and Synaptic Plasticity
J. Neurosci., July 15, 2000; 20(14): 5234 - 5244.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. Soares, Y. von Boxberg, M. Ravaille-Veron, J.-D. Vincent, and F. Nothias
Morphofunctional Plasticity in the Adult Hypothalamus Induces Regulation of Polysialic Acid-Neural Cell Adhesion Molecule through Changing Activity and Expression Levels of Polysialyltransferases
J. Neurosci., April 1, 2000; 20(7): 2551 - 2557.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.