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Glycobiology vol 5 no 8 pp. 797-806, 1995
© 1995

research-article

GlcNAc-transferase V and core 2 GlcNAc-transferase expression in the developing mouse embryo

M. Granovsky1, C. Fode, C.E. Warren2, R.M. Campbell1, J.D. Marth1, M. Pierce2, N. Fregien3 and J.W. Dennis4

Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue Toronto, Ontario, M5G 1X5 Department of Molecular and Medical Genetics, University of Toronto Toronto, Canada
1Howard Hughes Medical Institute, Division of Cellular and Molecular Medicine La Jolla, CA 192093, USA
2Department of Biochemistry, University of Georgia Athens, GA 30602, USA
3Department of Cell Biology and Anatomy, University of Miami Miami, FL, USA

4To whom correspondence should be addressed

Received on July 3, 1995; revised on August 21, 1995; accepted on September 2, 1995

UDP-GlcNAc:Man{alpha}1-6Manß-R ß1-6-N-acetylglucos-aminyltransferase V (GlcNAc-TV) and UDP-GlcNAc:Galß1-3GalNAc-R ß1-6-N-acetylglucosaminyltransferase (core 2 GlcNAc-T) are Golgi enzymes that catalyse the biosynthesis of ß1-6GlcNAc-branched intermediates in the N- and O-linked biosynthesis pathways, respectively. The activities of these enzymes change during haematopoiesis, embryo-carcinoma cell differentiation and following malignant transformation, but little is known about their expression in normal adult tissues and during embryogenesis. We have examined the expression of GlcNAc-TV and core 2 GlcNAc-T in sections of post-implantation mouse embryos by in situ RNA hybridization. The two enzymes showed distinct temporal and spatial patterns of expression. Core 2 GlcNAc-T mRNA was widely expressed at embryonic day (E) 7, and became restricted to a subset of mucin- and cartilage-producing tissues at E11.5 through to E17.5. GlcNAc-TV transcripts were absent at E7, became expressed throughout E9.5 embryos, and then progressively restricted to regions of the developing central nervous system and to specialized epithelia of skin, intestine, kidney, endocrine tissues and respiratory tract In the adult gonads, GlcNAc-TV transcripts were excluded from germ cells, but were detected in the follicular and testicular cells. Leukoagglutinin (L-PHA)-reactive oligosaccharides co-localized with GlcNAc-TV transcripts in skin, kidney and intestine, but brain showed unexpectedly low overall staining punctated by bright staining of the vascular endothelium. A common feature of cells in basal epithelia and in the cortical neural epithelium is the capacity to migrate, a cellular function which may require GlcNAc-TV-dependent glycoconjugates.

core 2 GlcNAc-transferase embryo expression GlcNAc-transferase V mouse


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