Skip Navigation


Glycobiology Advance Access originally published online on April 22, 2009
Glycobiology 2009 19(8):879-889; doi:10.1093/glycob/cwp062
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
19/8/879    most recent
cwp062v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Guillerme-Bosselut, F.
Right arrow Articles by Gallet, P.-F.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Guillerme-Bosselut, F.
Right arrow Articles by Gallet, P.-F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2009. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Glycosylation-related gene expression profiling in the brain and spleen of scrapie-affected mouse

Florence Guillerme-Bosselut2, Lionel Forestier2, Chantal Jayat-Vignoles3, Jean-Luc Vilotte4, Iuliana Popa5,6, Jacques Portoukalian5, Annick Le Dur7, Hubert Laude7, Raymond Julien2 and Paul-François Gallet1,2

2 INRA, UMR1061 Génétique Moléculaire Animale – Université de Limoges, F-87060 Limoges, France
3 CNRS, UMR 6101 Physiologie Moléculaire de la Réponse Immune et des Lymphoproliférations – Université de Limoges, F-87025 Limoges, France
4 INRA, UR1313 Génétique Animale et Biologie Intégrative, F-78350 Jouy-en-Josas, France
5 EA 4169 Université Lyon-1, Hopital Edouard Herriot, F-69000 Lyon, France
6 Institute of Macromolecular Chemistry, Iasi, Romania
7 INRA, UR892 Virologie Immunologie Moléculaires, F-78350 Jouy-en-Josas, France

1 To whom correspondence should be addressed: Tel: 335-5545-7654; Fax: 335-5545-7653; e-mail: francois.gallet{at}unilim.fr

Received on December 18, 2008; revised on April 17, 2009; accepted on April 18, 2009

A central event in the formation of infectious prions is the conformational change of a host-encoded glycoprotein, PrPC, into a pathogenic isoform, PrPSc. The molecular requirements for efficient PrP conversion remain unknown. Altered glycosylation has been linked to various pathologies and the N-glycans harbored by two prion protein isoforms are different. In order to search for glycosylation-related genes that could mark prion infection, we used a glycosylation-dedicated microarray that allowed the simultaneous analysis of the expression of 165 glycosylation-related genes encoding proteins of the glycosyltransferase, glycosidase, lectin, and sulfotransferase families to compare the gene expression profiles of normal and scrapie-infected mouse brain and spleen. Eight genes were found upregulated in "scrapie brain" at the final state of the disease. In the spleen, five genes presented a modified expression. Three genes were also upregulated in the spleen of infected mice, and two (Pigq and St3gal5) downregulated. All changes were confirmed by qPCR and biochemical analyses applied to Pigq and St3gal5 proteins.

Key words: brain / gene expression / microarray / prion disease / spleen


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.