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Glycobiology Advance Access originally published online on December 12, 2007
Glycobiology 2008 18(2):152-157; doi:10.1093/glycob/cwm134
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© The Author 2008. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Communication

Glucuronylation in Escherichia coli for the bacterial synthesis of the carbohydrate moiety of nonsulfated HNK-1

Elif Yavuz2, Sophie Drouillard2, Eric Samain2, Ian Roberts3 and Bernard Priem1,2

2 Centre de Recherches sur les Macromolécules Végétales, 601 rue de la Chimie, BP 53X, 38041 Grenoble, cedex 09, France
3 Faculty of Life Sciences, University of Manchester, Manchester, UK

1 To whom correspondence should be addressed: Fax: +33-476-547-203; e-mail: priem{at}cermav.cnrs.fr

Received on October 31, 2007; revised on December 3, 2007; accepted on December 4, 2007

We have previously reported the large-scale synthesis of neolactotetraose (Galβ-4GlcNAcβ-3Galβ-4Glc) from lactose in engineered Escherichia coli cells (Priem B, Gilbert M, Wakarchuk WW, Heyraud A and Samain E. 2002. A new fermentation process allows large-scale production of human milk oligosaccharides by metabolically engineered bacteria. Glycobiology. 12:235–240). In the present study we analyzed the adaptation of this system to glucuronylated oligosaccharides. The catalytic domain of mouse glucuronyl transferase GlcAT-P was cloned and expressed in an engineered strain which performed the in vivo synthesis of neolactotetraose. Under these conditions, efficient glucuronylation of neolactotetraose was achieved, but some residual neolactotetraose was still present. Although E. coli K-12 has an indigenous UDP-glucose dehydrogenase, the yield of glucuronylated oligosaccharides was greatly improved by the additional expression of the orthologous gene kfiD from E. coli K5. Glucuronylation of neolactohexaose and lactose was also observed. The final glucuronylated oligosaccharides are precursors of the brain carbohydrate motif HNK-1, involved in neural cell adhesion.

Key words: Escherichia coli / glucuronyltransferase / HNK-1 / metabolic engineering / UDP-glucose dehydrogenase


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