Skip Navigation


Glycobiology Advance Access originally published online on May 15, 2006
Glycobiology 2006 16(9):854-862; doi:10.1093/glycob/cwl001
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrowOA All Versions of this Article:
16/9/854    most recent
cwl001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (2)
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Duffy, M. S.
Right arrow Articles by Haslam, S. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Duffy, M. S.
Right arrow Articles by Haslam, S. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2006 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Protein glycosylation in Parelaphostrongylus tenuis—first description of the Gal{alpha}1-3Gal sequence in a nematode

Michael S. Duffy2, Howard R. Morris3, Anne Dell3, Judith A. Appleton2 and Stuart M. Haslam1,3

2 James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853; and 3 Division of Molecular Biosciences, Imperial College London, London SW7 2AZ, UK

1 To whom correspondence should be addressed; e-mail: s.haslam{at}imperial.ac.uk

Received on March 6, 2006; revised on May 9, 2006; accepted on May 10, 2006

The white-tailed deer is the definitive host of the parasitic nematode Parelaphostrongylus tenuis. This parasite also infects a wide variety of domesticated livestock, causing a debilitating neurologic disease. Glycoconjugates are becoming increasingly implicated in nematode strategies to maintain persistent infections in immunologically competent hosts. In this study, we have carried out detailed mass spectrometric analysis together with classical biochemical techniques, including western blotting and immunohistochemical staining with anticarbohydrate monoclonal antibodies and have shown that P. tenuis contains complex-type N-glycans with the antennae capped with Gal{alpha}1-3Galß1-4GlcNAc sequence. By mimicking a vertebrate glycan, Gal{alpha}1-3Gal may aid the parasite in evading immunological detection by the host. This is the first report of the Gal{alpha}1-3Gal sequence in a nematode.

Key words: glycosylation / Parelaphostrongylus tenuis / mass spectrometry / nematode


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.