Glycobiology Advance Access originally published online on December 8, 2005
Glycobiology 2006 16(4):281-293; doi:10.1093/glycob/cwj067
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Mass spectrometric approach for screening modifications of total serum N-glycome in human diseases: application to cirrhosis
2 Unité Mixte de Recherche CNRS/USTL 8576, Glycobiologie Structurale et Fonctionnelle, IFR 118, Université des Sciences et Technologies de Lille 1, 59655 Villeneuve d’Ascq, France; 3 Laboratoire de physiopathologie de la barrière hémato-encéphalique, E.A. 2465, IMPRT-IFR 114 Université d’Artois, rue Souvraz, SP18, 62307 Lens Cedex, France; 4 Services d’Hépato-Gastroentérologie, Hôpital Huriez, CHRU Lille, France; and 5 Laboratoire de Biochimie et de Biologie Moléculaire, UAM de glycopathologies, Hôpital Calmette, CHRU Lille, Bld du Professeur Jules Leclerc, Lille 59037 Cedex, France
1 To whom correspondence should be addressed; e-mail: a-klein{at}chru-lille.fr
Received on May 6, 2005; revised on December 5, 2005; accepted on December 6, 2005
Congenital and acquired modifications of glycosylation in diseases are a rapidly growing field that demonstrates the importance of glycosylation in human biology. Unfortunately, in clinical biochemistry, very few tests are available to explore oligosaccharide metabolism on a large scale. Such an assay needs to be of high throughput, rapid, and preferentially noninvasive. In the present study, we describe a method to analyze qualitative variations of N-glycosylation of human serum proteins. The method is based on direct release of N-linked oligosaccharides from patient serum samples, a single-step purification, and a matrix-assisted laser desorption ionization time of flight mass spectrometric analysis. A complementary structural study of the released oligosaccharides was achieved by enzymatic digestions, linkage analysis, and electrospray ionization ion trap mass spectrometry (ESI-IT-MS) of the permethylated N-glycome. A total of 26 oligosaccharide structures were individualized, their presence in human serum being the result of the combination of the biosynthesis and catabolic pathways. Application of the protocol to the serum of patients with cirrhosis demonstrates the ability of this assay to identify acquired modifications of glycosylation. Furthermore, we have analyzed the N-glycans and showed the increase in bisecting N-acetylglucosamine residue, core fucosylation, and the presence of an important population of neutral oligosaccharides. The study of total serum N-glycome modifications is a preliminary for the discovery of new noninvasive diagnostic or prognostic biomarkers resulting from the variations of the N-glycan metabolism during diseases.
Key words: cirrhosis / glycosylation disorders / mass spectrometry / N-glycan / N-glycome
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