Skip Navigation


Glycobiology Advance Access originally published online on August 11, 2005
Glycobiology 2005 15(12):1396-1406; doi:10.1093/glycob/cwj025
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
15/12/1396    most recent
cwj025v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (9)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Spirig, U.
Right arrow Articles by Aebi, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Spirig, U.
Right arrow Articles by Aebi, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oupjournals.org

The 3.4-kDa Ost4 protein is required for the assembly of two distinct oligosaccharyltransferase complexes in yeast

Urs Spirig1, Daniel Bodmer1, Michael Wacker, Patricie Burda and Markus Aebi2

Institute of Microbiology, ETH Zurich, CH-8093 Zurich, Switzerland

1 These authors contributed equally to this work.

2 To whom correspondence should be addressed; e-mail: aebi{at}micro.biol.ethz.ch

Received on July 4, 2005; accepted on July 29, 2005

In the central reaction of N-linked glycosylation, the oligosaccharyltransferase (OTase) complex catalyzes the transfer of a lipid-linked core oligosaccharide onto asparagine residues of nascent polypeptide chains in the lumen of the endoplasmic reticulum (ER). The Saccharomyces cerevisiae OTase has been shown to consist of at least eight subunits. We analyzed this enzyme complex, applying the technique of blue native gel electrophoresis. Using available antibodies, six different subunits were detected in the wild-type (wt) complex, including Stt3p, Ost1p, Wbp1p, Swp1p, Ost3p, and Ost6p. We demonstrate that the small 3.4-kDa subunit Ost4p is required for the incorporation of either Ost3p or Ost6p into the complex, resulting in two, functionally distinct OTase complexes in vivo. Ost3p and Ost6p are not absolutely required for OTase activity, but modulate the affinity of the enzyme toward different protein substrates.

Key words: blue native gel electrophoresis / N-linked protein glycosylation / oligosaccharyltransferase / Saccharomyces cerevisiae


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
F. Parsaie Nasab, B. L. Schulz, F. Gamarro, A. J. Parodi, and M. Aebi
All in One: Leishmania major STT3 Proteins Substitute for the Whole Oligosaccharyltransferase Complex in Saccharomyces cerevisiae
Mol. Biol. Cell, September 1, 2008; 19(9): 3758 - 3768.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
H. Kaji, J.-i. Kamiie, H. Kawakami, K. Kido, Y. Yamauchi, T. Shinkawa, M. Taoka, N. Takahashi, and T. Isobe
Proteomics Reveals N-Linked Glycoprotein Diversity in Caenorhabditis elegans and Suggests an Atypical Translocation Mechanism for Integral Membrane Proteins
Mol. Cell. Proteomics, December 1, 2007; 6(12): 2100 - 2109.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. M. Wilson and S. High
Ribophorin I acts as a substrate-specific facilitator of N-glycosylation
J. Cell Sci., February 15, 2007; 120(4): 648 - 657.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Chavan, Z. Chen, G. Li, H. Schindelin, W. J. Lennarz, and H. Li
Dimeric organization of the yeast oligosaccharyl transferase complex
PNAS, June 13, 2006; 103(24): 8947 - 8952.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
D. J. Kelleher and R. Gilmore
An evolving view of the eukaryotic oligosaccharyltransferase
Glycobiology, April 1, 2006; 16(4): 47R - 62R.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.