Skip Navigation


Glycobiology Advance Access originally published online on May 26, 2004
Glycobiology 2004 14(9):827-840; doi:10.1093/glycob/cwh100
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
14/9/827    most recent
cwh100v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (7)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Miyata, S.
Right arrow Articles by Kitajima, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miyata, S.
Right arrow Articles by Kitajima, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Glycobiology vol. 14 no. 9 © Oxford University Press 2004; all rights reserved.

A major flagellum sialoglycoprotein in sea urchin sperm contains a novel polysialic acid, an {alpha}2,9-linked poly-N-acetylneuraminic acid chain, capped by an 8-O-sulfated sialic acid residue

Shinji Miyata2, Chihiro Sato2,3, Shigeyuki Kitamura2, Masaru Toriyama4 and Ken Kitajima1,2,3,5

2 Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan; 3 Bioscience and Biotechnology Center, Nagoya University, Nagoya 464-8601, Japan; 4 Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Ohya, Shizuoka 422-8529, Japan; and 5 Institute for Advanced Research, Nagoya University, Nagoya 464-8601, Japan

Received on April 7, 2004; revised on May 15, 2004; accepted on May 21, 2004

A new type of polysialic acid (polySia) structure was demonstrated to occur in a major unknown sialoglycoprotein with a diverse molecular mass of 40–80 kDa in sea urchin sperm. The polySia-containing glycan structure was determined to be HSO3->8Neu5Ac{alpha}2->9(Neu5Ac{alpha}2->9)n–2 Neu5Ac{alpha}2->6GalNAc{alpha}1->Ser/Thr (n, on average 15), based on carbohydrate analysis of the sialoglycopeptide obtained by an exhaustive protease digestion of whole sperm, fluorometric anion-exchange high-performance liquid chromatography, and methylation analysis. The sulfate group was predominantly localized to the nonreducing terminus of the polySia chain. This is the first example of an {alpha}2,9-linked polySia structure in animal sperm. The polySia-containing sialoglycoprotein was present in sperm flagellum but not in the head. Furthermore, this sialoglycoprotein localized in the sperm lipid raft, which contains an enriched ganglioside (Neu5Ac{alpha}2->8Neu5Ac{alpha}2->6GlcCer), a receptor for sperm-activating peptide (speract), and its associated guanylate cyclase (Ohta et al. [2000] Glycoconj. J., 17, 205–214).

1 To whom correspondence should be addressed; e-mail: kitajima{at}agr.nagoya-u.ac.jp


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 GlycobiologyHome page
S. Miyata, C. Sato, H. Kumita, M. Toriyama, V. D. Vacquier, and K. Kitajima
Flagellasialin: a novel sulfated {alpha}2,9-linked polysialic acid glycoprotein of sea urchin sperm flagella
Glycobiology, December 1, 2006; 16(12): 1229 - 1241.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Nakata and F. A. Troy II
Degree of Polymerization (DP) of Polysialic Acid (PolySia) on Neural Cell Adhesion Molecules (N-CAMs): DEVELOPMENT AND APPLICATION OF A NEW STRATEGY TO ACCURATELY DETERMINE THE DP OF polySIA CHAINS ON N-CAMS
J. Biol. Chem., November 18, 2005; 280(46): 38305 - 38316.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.