Glycobiology Advance Access originally published online on June 9, 2004
Glycobiology 2004 14(9):757-766; doi:10.1093/glycob/cwh104
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Glycobiology vol. 14 no. 9 © Oxford University Press 2004; all rights reserved.
Engineering of an artificial glycosylation pathway blocked in core oligosaccharide assembly in the yeast Pichia pastoris: production of complex humanized glycoproteins with terminal galactose
GlycoFi, Inc., 21 Lafayette Street, Suite 200, Lebanon, NH 03766
Received on January 20, 2004; revised on May 25, 2004; accepted on May 26, 2004
A significant percentage of eukaryotic proteins contain posttranslational modifications, including glycosylation, which are required for biological function. However, the understanding of the structure–function relationships of N-glycans has lagged significantly due to the microheterogeneity of glycosylation in mammalian produced proteins. Recently we reported on the cellular engineering of yeast to replicate human N-glycosylation for the production of glycoproteins. Here we report the engineering of an artificial glycosylation pathway in Pichia pastoris blocked in dolichol oligosaccharide assembly. The PpALG3 gene encoding Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase was deleted in a strain that was previously engineered to produce hybrid GlcNAcMan5GlcNAc2 human N-glycans. Employing this approach, combined with the use of combinatorial genetic libraries, we engineered P. pastoris strains that synthesize complex GlcNAc2Man3GlcNAc2 N-glycans with striking homogeneity. Furthermore, through expression of a Golgi-localized fusion protein comprising UDP-glucose 4-epimerase and ß-1,4-galactosyl transferase activities we demonstrate that this structure is a substrate for highly efficient in vivo galactose addition. Taken together, these data demonstrate that the artificial in vivo glycoengineering of yeast represents a major advance in the production of glycoproteins and will emerge as a practical tool to systematically elucidate the structure–function relationship of N-glycans.
1 These authors contributed equally to this work.
2 To whom correspondence should be addressed; e-mail: swildt{at}glycofi.com
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