Skip Navigation


Glycobiology Advance Access originally published online on July 14, 2004
Glycobiology 2004 14(12):1275-1284; doi:10.1093/glycob/cwh128
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
14/12/1275    most recent
cwh128v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (13)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Cesaretti, M.
Right arrow Articles by Volpi, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cesaretti, M.
Right arrow Articles by Volpi, N.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Glycobiology vol. 14 no. 12 © Oxford University Press 2004; all rights reserved.

Isolation and characterization of a heparin with high anticoagulant activity from the clam Tapes phylippinarum: evidence for the presence of a high content of antithrombin III binding site

Marina Cesaretti, Elisa Luppi, Francesca Maccari and Nicola Volpi1

Department of Biologia Animale, University of Modena and Reggio Emilia, Via Campi 213/D, 41100 Modena, Italy

Received on May 6, 2004; revised on July 6, 2004; accepted on July 7, 2004

Heparin with high anticoagulant activity (activated partial thromboplastin time of 347 ± 56.4 and anti-Xa activity of 317 ± 48.3) was isolated from the marine clam species Tapes phylippinarum in an amount of ~2.1 mg/g dry animals. Agarose-gel electrophoresis showed a high content of the slow-moving heparin component (22 ± 6.8%) and 78 ± 5.4% of the fast-moving species. An average molecular mass of 13,600 was calculated by PAGE analysis, whereas a number average molecular weight Mn value of 10,700, a weight average molecular weight Mw of 14,900, and a dispersity index Mn/Mw of 1.386 were obtained by high-performance size-exclusion chromatography. Structural analysis of clam heparin, performed by depolymerizing heparin samples with heparinase (EC 4.2.2.7) and then separating the resulting unsaturated oligosaccharides by strong anion exchange–HPLC revealed the presence of large amounts (more than 130% than standard pharmaceutical heparin obtained from bovine intestine) of the oligosaccharide sequence bearing part of the ATIII-binding region, {Delta}UA2S (1->4)-{alpha}-D-GlcN2S6S (1->4)-{alpha}-L-IdoA (1->4)-{alpha}-D-GlcNAc6S (1->4)-ß-D-GlcA (1->4)-{alpha}-D-GlcN2S3S6S in the T. phylippinarum heparin, in comparison with bovine mucosal heparin and a sample of porcine mucosal heparin previously published. Furthermore, as expected from the oligosaccharide compositional analysis, due to the presence of a great mol % (80.6%) of the trisulfated disaccharide {Delta}UA2S(1->4)-{alpha}-D-GlcN2S6S, mollusc heparin is a more sulfated polysaccharide than bovine mucosal heparin (73.5%) and a sample of porcine mucosal (72.8%) heparin previously reported. To our knowledge, this is the first article describing a clam heparin having the ATIII binding site mainly identical to that of human and porcine intestinal mucosal heparins and bovine intestinal mucosal heparin but different from that found in beef lung heparin.

1 To whom correspondence should be addressed; e-mail: volpi{at}unimo.it


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 GlycobiologyHome page
N. Volpi and F. Maccari
Structural characterization and antithrombin activity of dermatan sulfate purified from marine clam Scapharca inaequivalvis
Glycobiology, April 1, 2009; 19(4): 356 - 367.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Lawrence, S. K. Olson, R. E. Steele, L. Wang, R. Warrior, R. D. Cummings, and J. D. Esko
Evolutionary Differences in Glycosaminoglycan Fine Structure Detected by Quantitative Glycan Reductive Isotope Labeling
J. Biol. Chem., November 28, 2008; 283(48): 33674 - 33684.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
H. Xu, T. Guo, Y.-f. Guo, J. e. Zhang, Y. Li, W. Feng, and B. Jiao
Characterization and Protection on Acute Liver Injury of a Polysaccharide MP-I from Mytilus Coruscus
Glycobiology, January 1, 2008; 18(1): 97 - 103.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Sinnis, A. Coppi, T. Toida, H. Toyoda, A. Kinoshita-Toyoda, J. Xie, M. M. Kemp, and R. J. Linhardt
Mosquito Heparan Sulfate and Its Potential Role in Malaria Infection and Transmission
J. Biol. Chem., August 31, 2007; 282(35): 25376 - 25384.
[Abstract] [Full Text] [PDF]

Home page
 CLIN APPL THROMB HEMOSTHome page
W. P. Jeske, M. K. McDonald, D. A. Hoppensteadt, E. C. Bau, A. Mendes, C. P. Dietrich, J. M. Walenga, and E. Coyne
Isolation and Characterization of Heparin From Tuna Skins
Clinical and Applied Thrombosis/Hemostasis, April 1, 2007; 13(2): 137 - 145.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
C. M. de Barros, L. R. Andrade, S. Allodi, C. Viskov, P. A. Mourier, M. C. M. Cavalcante, A. H. Straus, H. K. Takahashi, V. H. Pomin, V. F. Carvalho, et al.
The Hemolymph of the Ascidian Styela plicata (Chordata-Tunicata) Contains Heparin inside Basophil-like Cells and a Unique Sulfated Galactoglucan in the Plasma
J. Biol. Chem., January 19, 2007; 282(3): 1615 - 1626.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.