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Glycobiology Advance Access originally published online on July 8, 2003
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Glycobiology, 2003, Vol. 13, No. 11 749-754
© 2003 Oxford University Press

A new kind of carbohydrate array, its use for profiling antiglycan antibodies, and the discovery of a novel human cellulose-binding antibody

Mikael Schwarz1,, Larissa Spector, Ari Gargir, Avraham Shtevi, Monica Gortler, Rom T. Altstock, Avinoam A. Dukler and Nir Dotan

Glycominds Ltd., 1 Yodfat Street, Alon Bldg., Lod 71291, Israel

Received on March 3, 2003; revised on May 8, 2003; accepted on May 30, 2003

In this study, we use a novel glycan array to analyze the glycan-binding antibody repertoire in a pool of affinity-purified IgG collected from a healthy human population. The glycan array used is based on mono- and oligosaccharides covalently linked to the surface via a long linker at their reducing ends. They are thus presented to the medium with a well-defined orientation and are accessible for specific binding by glycan-binding proteins, such as antibodies and lectins. A novel anticellulose antibody was detected that binds specifically to ß4-linked saccharides with a preference for glucopyranose over galactopyranose residues. We also found previously known antiglycan antibodies against mono- and oligosaccharides that are constituents of commonly occurring bacterial polysaccharides. We propose that this array can facilitate high-throughput screening of glycan-binding proteins and the search for biomarkers for personalized medicine.

1 To whom correspondence should be addressed; email: mikaels{at}glycominds.com


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