Glycobiology Advance Access originally published online on July 8, 2003
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Glycobiology, 2003, Vol. 13, No. 10 681-692
© 2003 Oxford University Press
ß-Galactofuranose-containing O-linked oligosaccharides present in the cell wall peptidogalactomannan of Aspergillus fumigatus contain immunodominant epitopes
3 Instituto de Microbiologia Professor Paulo de Góes, CCS Universidade Federal do Rio de Janeiro (UFRJ), 21944-970, Rio de Janeiro, RJ, Brasil; 4 Instituto Biomedico, Universidade do Rio de Janeiro, 20211-040, Rio de Janeiro, RJ, Brasil; 5 Centro Nacional de Ressonância Magnética Nuclear, Departamento de Bioquímica, ICB/UFRJ, 21941-590, Rio de Janeiro, RJ, Brasil; 6 Institute for Medical Physics and Biophysics, University of Münster, Robert-Koch Str 31, D-48149, Münster, Germany; 7 University of Bielefeld, Bielefeld, Germany; and 8 Departamento de Bioquímica, Universidade Federal do Paraná (UFPR), D-33501, 81531-990, Curitiba, Paraná, Brazil
Received on January 16, 2003; revised on May 28, 2003; accepted on May 30, 2003
O-linked oligosaccharide groups ranging from di- to hexasaccharide were ß-eliminated by mild alkaline treatment under reducting conditions from the peptidogalactomannan of Aspergillus fumigatus mycelial cell wall. The resulting reduced oligosaccharides, which were the minor components of the peptidogalactomannan fraction, were fractionated to homogeneity by successive gel filtration and high-performance liquid chromatography. Their primary structures were determined based on a combination of techniques including gas chromatography, ESI-QTOF-MS, 1H COSY and TOCSY, and 1H-13C HMQC NMR spectroscopy and methylation analysis, to be: 
-Glcp-(1 
6)-Man-ol, ß-Galf-(1 6)--Manp-(1 6)-Man-ol, ß-Galf-(1 5)-ß-Galf-(1 6)--Manp-(1 6)-Man-ol and ß-Galf-(1 5)-[ß-Galf-(1 5]3-ß-Galf-(1 6)-Man-ol. The ß-Galf containing oligosaccharides have not been previously described as fungal O-linked oligosaccharides. The peptidogalactomannan is antigenic and was recognized by human sera of patients with aspergillosis when probed by ELISA, but de-O-glycosylation rendered a 50% decrease in its reactivity. Furthermore, when tested in a hapten inhibition test, the isolated oligosaccharide alditols were able to block, on a dose-response basis, recognition between human sera and the intact peptidogalactomannan. The immunodominant epitopes were present in the tetra- and hexasaccharide, which contain a ß-Galf-(1 5)-ß-Galf terminal group. These results suggest that the O-glycosidically linked oligosaccharide chains, despite being the less abundant carbohydrate component of the A. fumigatus peptidogalactomannan, may account for a significant part of its antigenicity, other than the known activity associated with the galactomannan component.
2 To whom correspondence should be addressed; email: eliana.bergter{at}micro.ufrj.br
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