Glycobiology Advance Access originally published online on November 1, 2002
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Glycobiology, 2003, Vol. 13, No. 1 1G-3G
© 2003 Oxford University Press
GLYCO-FORUM SECTION |
Alternative sources of reagents and supplies for fluorophore-assisted carbohydrate electrophoresis (FACE)
Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390-9041, USA
Received on September 3, 2002; revised on September 6, 2002; accepted on September 6, 2002
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Introduction
Fluorophore-assisted carbohydrate electrophoresis (FACE) is a simple, sensitive, and versatile method for analysis of both monosaccharides and oligosaccharides and has become an important method in glycobiology. Recently, the major provider of reagents and supplies for FACE has ceased production and shipping. To assist those who have relied on this provider for FACE reagents and supplies, the editors have asked us to summarize alternative commercial sources and procedures that we have used (Gao and Lehrman, 2002a
,b). Our procedures are modified from those published previously (Jackson, 1994; Starr et al., 1996).
Oligosaccharide profiling gels
Reagents
ANTS (8-aminonophthalene-1,3,6-trisulfonate, catalog A-350) and AMAC (2-aminoacridone, A-6289) are from Molecular Probes (Eugene, OR). ANDS (7-amino-1,3-naphthalenedisulfonic acid, 14644-7) and sodium cyanoborohydride (15615-9) are from Aldrich Chemical (Milwaukee, WI).
Fluorescent oligosaccharide standards
A mild acid hydrolysate (0.1 M HCl,
Gel electrophoresis apparatus
Imager
Derivatization of oligosaccharides with ANTS andANDS
Preparation of polyacrylamide gels for oligosaccharide profiling
Electrophoretic separation of ANTS- and ANDS-labeled oligosaccharides
Monosaccharide composition gels
Derivatization of monosaccharides with AMAC
Preparation of polyacrylamide gels for monosaccharide composition
Electrophoretic separation of AMAC-labeled monosaccharides
1 To whom correspondence should be addressed; e-mail: mark.lehrman@utsouthwestern.edu 
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