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Glycobiology, 2002, Vol. 12, No. 7 435-442
© 2002 Oxford University Press

Molecular cloning, gene organization, and expression of mouse Mpi encoding phosphomannose isomerase

Joseph A. Davis, Xiao-Hua Wu1, Ling Wang1, Charles DeRossi, Vibeke Westphal, Rongrong Wu, Gordon Alton, Geetha Srikrishna and Hudson H. Freeze2

Glycobiology Program, The Burnham Institute, 10901 North Torrey Pines Rd., La Jolla, CA 92037, USA

Phosphomannose isomerase (PMI) interconverts fructose-6-P (Fru-6-P) and mannose-6-P (Man-6-P), linking energy metabolism to protein glycosylation. We have cloned the mouse Mpi cDNA, analyzed its genomic organization, and studied the expression in different tissues. The Mpi gene has eight exons covering 7.2 kb. The structure and intron–exon boundaries are essentially the same as its human ortholog with 85% amino acid identity. Mpi is alternatively spliced at the 3' end, resulting in three messages with different 3'-untranslated regions. Mpi expression is regulated at both the transcription and translation levels, with the highest expression level in testis. Rabbit antibodies prepared against mouse PMI expressed in E. coli recognize a single 47-kDa band. Immunohistochemistry of mouse tissues shows general cytosolic staining in all cells. In testis, staining is intense in round spermatids and residual bodies, moderate in pachytene spermatocytes, and weak in spermatogonia and spermatozoa. In contrast, northern blot analysis shows comparable transcripts of 1.8 and 1.6 kb in pachytene spermatocytes and round spermatids, suggesting delayed translation of PMI. The stage-specific expression of PMI in testis may be important for KDN synthesis, which requires Man-6-P, or it may be needed to ensure sufficient glycosylation precursors in cells that do not utilize glucose and instead rely on lactate and pyruvate.

1 These authors contributed equally to this work.

2 To whom correspondence should be addressed; E-mail: hudson{at}burnham.org


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C. DeRossi, L. Bode, E. A. Eklund, F. Zhang, J. A. Davis, V. Westphal, L. Wang, A. D. Borowsky, and H. H. Freeze
Ablation of Mouse Phosphomannose Isomerase (Mpi) Causes Mannose 6-Phosphate Accumulation, Toxicity, and Embryonic Lethality
J. Biol. Chem., March 3, 2006; 281(9): 5916 - 5927.
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