Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (12)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Inoue, S.
Right arrow Articles by Inoue, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Inoue, S.
Right arrow Articles by Inoue, Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Glycobiology, 2001, Vol. 11, No. 9 759-767
© 2001 Oxford University Press

An ultrasensitive chemical method for polysialic acid analysis

Sadako Inoue1,2, Shu-Ling Lin2, Yuan Chuan Lee3 and Yasuo Inoue2

2Institute of Biological Chemistry, Academia Sinica, Taipei 11529, Taiwan, ROC, and 3Biology Department, Johns Hopkins University, Baltimore, Maryland 21218, USA

An ultrasensitive method for analysis of polysialic acid (polySia) chains, using fluorescence-assisted high-performance liquid chromotography was developed. The new method is a substantial improvement of our earlier method in which the reducing terminal Sia residues of a homologous series of oligo/polySia hydrolytically released during derivatization reaction were simultaneously labeled with a fluorogenic reagent, 1,2-diamino-4,5-methylenedioxybenzene (DMB) in situ. We first studied extensively the stability of oligo/polySia in the acid (0.02 M trifluoracetic acid) used for 1,2-diamino-4,5-methylenedioxybenzene derivatization under various conditions of reaction time and temperature, analyzing the hydrolytic products by high-performance anion exchange chromatography with pulsed electrochemical detection (HPAEC-PED). Then we optimized the reaction conditions to minimize degradation of the parent polySia while maintaining high derivatization rate. Using a DNAPac PA-100 column rather than a MonoQ column, baseline resolution of polySia peaks up to DP 90 with a detection threshold of 1.4 femtomol per resolved peak was achieved. The new method was used to analyze the degree of polymerization of a polySia-containing glycopeptide fraction derived from embryonic chicken brain, and the results were compared with those obtained by HPAEC-PED.

1 To whom correspondence should be addressed


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S. P. Galuska, R. Geyer, R. Gerardy-Schahn, M. Muhlenhoff, and H. Geyer
Enzyme-dependent Variations in the Polysialylation of the Neural Cell Adhesion Molecule (NCAM) in Vivo
J. Biol. Chem., January 4, 2008; 283(1): 17 - 28.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
J. Vionnet and W. F Vann
Successive glycosyltransfer of sialic acid by Escherichia coli K92 polysialyltransferase in elongation of oligosialic acceptors
Glycobiology, July 1, 2007; 17(7): 735 - 743.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Nakata and F. A. Troy II
Degree of Polymerization (DP) of Polysialic Acid (PolySia) on Neural Cell Adhesion Molecules (N-CAMs): DEVELOPMENT AND APPLICATION OF A NEW STRATEGY TO ACCURATELY DETERMINE THE DP OF polySIA CHAINS ON N-CAMS
J. Biol. Chem., November 18, 2005; 280(46): 38305 - 38316.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
Y. C. Lee
Letter to the Glyco-Forum: A Green Thumb and a Broad Back: A Tribute to the late Dr. Yasuo Inoue (1934-2005)
Glycobiology, May 1, 2005; 15(5): 9G - 11G.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Inoue, G. L. Poongodi, N. Suresh, H. J. Jennings, and Y. Inoue
Discovery of an alpha 2,9-PolyNeu5Ac Glycoprotein in C-1300 Murine Neuroblastoma (Clone NB41A3)
J. Biol. Chem., February 28, 2003; 278(10): 8541 - 8546.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. L. Poongodi, N. Suresh, S. C. B. Gopinath, T. Chang, S. Inoue, and Y. Inoue
Dynamic Change of Neural Cell Adhesion Molecule Polysialylation on Human Neuroblastoma (IMR-32) and Rat Pheochromocytoma (PC-12) Cells during Growth and Differentiation
J. Biol. Chem., July 26, 2002; 277(31): 28200 - 28211.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.