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Glycobiology, 2001, Vol. 11, No. 11 969-977
© 2001 Oxford University Press

Identification of a nonmucin glycoprotein (gp-340) from a purified respiratory mucin preparation: evidence for an association involving the MUC5B mucin

David J. Thornton1,2, Julia R. Davies3, Sara Kirkham2, Alex Gautrey2, Nagma Khan2, Paul S. Richardson4 and John K. Sheehan2

2Wellcome Trust Centre for Cell-Matrix Research, Division of Biochemistry, School of Biological Sciences, 2.205 Stopford Building, University of Manchester, Manchester, M13 9PT, UK; 3Mucosal Biology Group, Department of Cell and Molecular Biology, Lund University, BMC, C-13, SE-22184, Lund, Sweden; 4St. George’s Hospital Medical School, Department of Physiology, Cranmer Terrace, London, SW17 ORE, UK.

Rate-zonal centrifugation of a reduced and alkylated respiratory mucin preparation identified a protein-rich fraction. This was subjected to trypsin treatment and one of the many liberated peptides was purified and its N-terminal sequence determined. The peptide was identical to a 14 amino acid sequence from the scavenger receptor cysteine-rich domain containing glycoprotein gp-340. A polyclonal antiserum, raised against the peptide, stained the serous cells in the submucosal glands of human tracheal tissue. The glycoprotein was purified from respiratory mucus by density-gradient centrifugation, gel chromatography, and anion exchange chromatography. The molecule exhibited a heterogeneous distribution of buoyant density (1.28–1.46 g/ml) that overlapped with the gel-forming mucins, was included on Sepharose CL-2B and was quite highly anionic. SDS–PAGE indicated a mass greater than 208 kDa and measurements performed across the molecular size distribution indicated an average Mr of 5 x 105 with a range of Mr from 2 x 105 to 1 x 106. Gel chromatography of respiratory mucus extracts ("associative" and "dissociative") indicated that this glycoprotein forms complexes that may involve the large gel-forming mucins MUC5AC and MUC5B. Rate zonal centrifugation suggested such complexes are more likely to involve MUC5B rather than MUC5AC mucins.

1 To whom correspondence should be addressed


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