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Glycobiology, 2000, Vol. 10, No. 9 941-950
© 2000 Oxford University Press

Phosphorylcholine-containing N-glycans of Trichinella spiralis: identification of multiantennary lacdiNAc structures

Willy Morelle, Stuart M.Haslam, Verena Olivier1,3, Judith A. Appleton3,4, Howard R. Morris and Anne Dell2

Department of Biochemistry, Imperial College, London, SW7 2AY, UK and 3James A.Baker Institute for Animal Health and 4Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA

Although the presence of phosphorylcholine (PC) in Trichinella spiralis is well established, the precise structure of the PC-bearing molecules is not known. In this paper, we report structural studies of N-glycans released from T.spiralis affinity-purified antigens by peptide N-glycosidase F. Three classes of N-glycan structures were observed: high mannose type structures; those which had been fully trimmed to the trimannosyl core and were sub-stoichiometrically fucosylated; and those with a trimannosyl core, with and without core fucosylation, carrying between one and eight N-acetylhexosamine residues. Of the three classes of glycans, only the last was found to be substituted with detectable levels of phosphorylcholine.

1 Present address: University of Muenster, Muenster, Germany.

2 To whom correspondence should be addressed


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