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Glycobiology, 2000, Vol. 10, No. 2 185-192
© 2000 Oxford University Press

Urinary excretion of glycosaminoglycans and albumin in experimental diabetes mellitus

Ricardo A. M. Cadaval, Oswaldo Kohlman and Yara M. Michelacci1,2

Disciplina de Nefrologia, Departamento de Medicina, and 2Disciplina de Biologia Molecular, Departamento de Bioquímica, Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, Brazil

Diabetes mellitus was induced in one group of rats by a single injection of streptozotocin. The glycemia, the body weight, and the blood systolic pressure were measured every week, and the 24 h urine volume and urinary excretions of creatinine, albumin and glycosaminoglycans were measured every 2 weeks. At the end of the experiment (12 weeks) the weight and the glycosaminoglycan composition of the kidneys were determined. All the diabetic animals were hyperglycemic, hypertense, and did not gain weight during all the experimental period. Albuminuria appeared from the second week on. Rat urine was shown to contain heparan sulfate, chondroitin sulfate, and dermatan sulfate, and the glycosaminoglycan excretion decreased in all diabetic animals. The onset of the change in glyco­samino­glycan excretion rate was a very early event, appearing in the second week after diabetes induction. The main glycosaminoglycan found in normal rat kidney was heparan sulfate and, in contrast to the urine, the total kidney glycosaminoglycans increased in diabetic kidney, due to chondroitin sulfate and dermatan sulfate accumulation. The heparan sulfate concentration (per tissue dry weight) did not change. Our results suggest that quantification of urinary glycosaminoglycans may be a useful tool for the early diagnosis of diabetic nephropathy.

1 To whom correspondence should be addressed at: Disciplina de Biologia Molecular, Departamento de Bioquímica, UNIFESP/EPM, Universidade Federal de São Paulo/Escola Paulista de Medicina, Rua Três de Maio 100, 04023–020 São Paulo, Brazil


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