Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (11)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by O’Riordan, C. R.
Right arrow Articles by Cheng, S. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by O’Riordan, C. R.
Right arrow Articles by Cheng, S. H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Glycobiology, 2000, Vol. 10, No. 11 1225-1233
© 2000 Oxford University Press

Characterization of the oligosaccharide structures associated with the cystic fibrosis transmembrane conductance regulator

Catherine R. O’Riordan1, Amy L. Lachapelle, John Marshall, Elizabeth A. Higgins and Seng H. Cheng

Genzyme Corporation, 31 New York Avenue, Framingham, MA 01701–9322, USA

The cystic fibrosis transmembrane conductance regulator (CFTR) is a plasma membrane-associated glycoprotein. The protein can exist in three different molecular weight forms of approximately 127, 131, and 160 kDa, representing either nonglycosylated, core glycosylated, or fully mature, complex glycosylated CFTR, respectively. The most common mutation in cystic fibrosis (CF) results in the synthesis of a variant ({Delta}F508-CFTR) that is incompletely glycosylated and defective in its trafficking to the cell surface. In this study, we have analyzed the oligosaccharide structures associated with the different forms of recombinant CFTR, by expressing and purifying the channel protein from either mammalian Chinese hamster ovary (CHO) or insect Sf9 cells. Using glycosidases and FACE analysis (fluorophore-assisted carbohydrate electrophoresis) we determined that purified CHO-CFTR contained polylactosaminoglycan (PL) sequences, while Sf9-CFTR had only oligomannosidic saccharides with fucosylation on the innermost GlcNAc. The presence of PL sequences on the recombinant CHO-CFTR is consistent with a normal feature of mammalian processing, since endogenous CFTR isolated from T84 cells displayed a similar pattern of glycosylation. The present study also reports on the use of FACE for the qualitative analysis of small amounts of glycoprotein oligosaccharides released enzymatically.

1 To whom correspondence should be addressed


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
K. Mio, T. Ogura, M. Mio, H. Shimizu, T.-C. Hwang, C. Sato, and Y. Sohma
Three-dimensional Reconstruction of Human Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channel Revealed an Ellipsoidal Structure with Orifices beneath the Putative Transmembrane Domain
J. Biol. Chem., October 31, 2008; 283(44): 30300 - 30310.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. N. Mikerov, G. Wang, T. M. Umstead, M. Zacharatos, N. J. Thomas, D. S. Phelps, and J. Floros
Surfactant Protein A2 (SP-A2) Variants Expressed in CHO Cells Stimulate Phagocytosis of Pseudomonas aeruginosa More than Do SP-A1 Variants
Infect. Immun., March 1, 2007; 75(3): 1403 - 1412.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
F. Jouret, A. Bernard, C. Hermans, G. Dom, S. Terryn, T. Leal, P. Lebecque, J.-J. Cassiman, B. J. Scholte, H. R. de Jonge, et al.
Cystic Fibrosis Is Associated with a Defect in Apical Receptor-Mediated Endocytosis in Mouse and Human Kidney
J. Am. Soc. Nephrol., March 1, 2007; 18(3): 707 - 718.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. L. de Graffenried and C. R. Bertozzi
Golgi Localization of Carbohydrate Sulfotransferases Is a Determinant of L-selectin Ligand Biosynthesis
J. Biol. Chem., October 10, 2003; 278(41): 40282 - 40295.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Jin, T. M. Weiger, and I. B. Levitan
Reciprocal Modulation between the alpha and beta 4 Subunits of hSlo Calcium-dependent Potassium Channels
J. Biol. Chem., November 8, 2002; 277(46): 43724 - 43729.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-S. Yoo, B. D. Moyer, S. Bannykh, H.-M. Yoo, J. R. Riordan, and W. E. Balch
Non-conventional Trafficking of the Cystic Fibrosis Transmembrane Conductance Regulator through the Early Secretory Pathway
J. Biol. Chem., March 22, 2002; 277(13): 11401 - 11409.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.