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Glycobiology vol 1 no 2 pp. 163-171, 1991
© 1991

research-article

Characterization of nematode glycoproteins: the major O-glycans of Toxocara excretory-secretory antigens are O-methylated trisaccharides

Kay-Hooi Khoo, Rick M. Maizels1, Anthony P. Page1, Graham W. Taylor2, Nigel B. Rendell2 and Anne Dell3

Departments of Biochemistry London SW7 2AZ
1Biology, Imperial College of Science, Technology and Medicine London SW7 2AZ
2Department of Clinical Pharmacology, Royal Postgraduate Medical School Du Cane Road, London W12 0NN, UK

3To whom correspondence should be addressed

Received on October 5, 1990; accepted on October 22, 1990

Toxocara excretory-secretory antigens (TES) were isolated from the culture media of T.canis and T.cati larvae and their O-glycan content was investigated using fast atom bombardment-mass spectrometry (FAB-MS), gas chromatography and electron impact mass spectrometry. The major oligosaccharides released by reductive elimination of T.canis TES glycoproteins were shown to be two, approximately equi-abundant, trisaccharides 2-O-Me-Fucp({alpha}1->2)-4-O-Me-Galp(ß1->3)GalNAcitol and 2-O-Me-Fucp({alpha}1->2)-Galp(ß1->3)GalNAcitol. In contrast T.cati TES O-glycans are predominantly one component, shown by FAB-MS to be a di-O-methylated trisaccharide, which is probably identical to the di-O-methylated trisaccharide from T.canis. The O-methylated trisaccharides are strong candidates for the carbohydrate epitopes recognized by a panel of monoclonal antibodies which exhibit multiple reactivity against TES antigens. This study constitutes the first rigorous characterization of glycans from a parasitic nematode.

glyco-antigens FAB-MS O-methylated trisaccharides nematode glycoproteins structure analysis


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