Glycobiology 2008 18(5):352; doi:10.1093/glycob/cwn025
Published by Oxford University Press 2008.
Erratum
Free oligosaccharide regulation during mammalian protein N-glycosylation
doi:10.1093/glycob/cwn003
Glycobiology vol. 18 no. 3 pp. 210–224, 2008
Figure 3 and its caption should have appeared as follows:
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Fig. 3 Two Png1-independent fOS pools are detected in yeast. S. cerevisae strains lacking Png1p (png1 ) and the proton pumping subunit of the vacuolar H+/ATPase (vma1), and an isogenic wild-type strain (wt) were pulse radiolabeled with [2-3H]Man for 30 min and then chased for the indicated times as previously described (Chantret I, Frenoy JP, et al. 2003). fOS were isolated, resolved by thin layer chromatography, and revealed by fluorography. Shorter exposures of lanes 1 and 4 have been published previously (Chantret I, Frenoy JP, et al. 2003). Abolishing S. cerevisiae Png1p activity greatly reduces but does not eliminate fOS generation, and at least two Png1p-independent fOS pools are observed. The first, more abundant, Png1-independent fOS pool comprises predominantly the structure Man8GlcNAc2, but in contrast to the Png1p-dependent pool, has a short half-life and disappears without the appearance of intermediates. The second Png1p-independent pool comprises smaller [2-3H]Man-labeled components (see asterisks) the most abundant of which comigrates with a Man3GlcNAc2 standard. The metabolism of this fOS pool is clearly altered in the yeast strain deficient in vacuolar acidification.
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