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Glycobiology Advance Access published online on October 8, 2009
Glycobiology, doi:10.1093/glycob/cwp161
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© 2009 The Author(s). Published by Oxford University Press. All rights reserved.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses?by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

MUC2 mucin is a major carrier of the cancer-associated sialyl-Tn antigen in intestinal metaplasia and gastric carcinomas

Tim Conze1,*, Ana Sofia Carvalho2,*, Ulf Landegren1, Raquel Almeida2,3, Celso A. Reis2,3, Leonor David2,3 and Ola Söderberg1

1 Department of Genetics and Pathology, University of Uppsala, Uppsala, Sweden
2 Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Porto, Portugal
3 Medical Faculty of the University of Porto, Porto, Portugal

Correspondence, Ola Söderberg, Department of Genetics and Pathology, Rudbeck laboratory, University of Uppsala, 751 85 Uppsala, Sweden, Phone: +46 18 4714868, E-mail: ola.soderberg{at}genpat.uu.se

Received on June 9, 2009; accepted on October 5, 2009

Changes in mucin protein expression and in glycosylation are common features in pre-neoplastic lesions and cancer and are therefore used as cancer-associated markers. De novo expression of intestinal mucin MUC2 and cancer-associated sialyl-Tn antigen are frequently observed in intestinal metaplasia (IM) and gastric cancer. However, despite that these antigens often co-localize, MUC2 has not been demonstrated to be a carrier of sialyl-Tn. By using the in situ proximity ligation assay (in situ PLA) we herein could show that MUC2 is a major carrier of the sialyl-Tn antigen in all IM cases and in most gastric carcinoma cases. The requirement by in situ PLA for the presence of both antigens in close proximity increases the selectivity compared to measurement of co-localization, as determined by immunohistochemistry. To identify the mucin which is the carrier of a carbohydrate structure offers unique advantages for future development of more accurate diagnostic and prognostic markers.

Key words: Gastric cancer / Glycosylation / In situ proximity ligation assay / Intestinal metaplasia / Sialyl-Tn

* These authors contributed equally to this work.


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