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Glycobiology Advance Access published online on September 30, 2009
Glycobiology, doi:10.1093/glycob/cwp157
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© The Author 2009. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

An {alpha}2,6-sialyltransferase cloned from Photobacterium leiognathi strain JT-SHIZ-119 shows both sialyltransferase and neuraminidase activity

Toshiki Mine, Sakurako Katayama, Hitomi Kajiwara, Masako Tsunashima, Hiroshi Tsukamoto, Yoshimitsu Takakura and Takeshi Yamamoto1

Glycotechnology Business Unit, Japan Tobacco Inc., 700 Higashibara, Iwata, Shizuoka 438-0802, JAPAN

1 To whom correspondence should be addressed: Tel: +81-538-32-7389; Fax: +81-538-33-6046; e-mail: takeshi.yamamoto{at}jt.com

Received on July 29, 2009; accepted on September 28, 2009

We cloned, expressed, and characterized a novel β-galactoside {alpha}2,6-sialyltransferase from Photobacterium leiognathi strain JT-SHIZ-119. The protein showed 56% to 96% identity to the marine bacterial {alpha}2,6-sialyltransferases classified into glycosyltransferase family 80. The sialyltransferase activity of the N-terminal truncated form of the recombinant enzyme was 1477 U/L of Escherichia coli culture. The truncated recombinant enzyme was purified as a single band by sodium dodecyl sulfate polyacrylamide gel electrophoresis through 3 column chromatography steps. The enzyme had distinct activity compared with known marine bacterial {alpha}2,6-sialyltransferases. Although {alpha}2,6-sialyltransferases cloned from marine bacteria, such as Photobacterium damselae strain JT0160, P. leiognathi strain JT-SHIZ-145, and Photobacterium sp. strain JT-ISH-224, show only {alpha}2,6-sialyltransferase activity, the recombinant enzyme cloned from P. leiognathi strain JT-SHIZ-119 showed both {alpha}2,6-sialyltransferase and {alpha}2,6-linkage-specific neuraminidase activity. Our results provide important information toward a comprehensive understanding of the bacterial sialyltransferases belonging to the group 80 glycosyltransferase family in the CAZy database.

Key words: bi-functional sialyltransferase / neuraminidase / Photobacterium leiognathi


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