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Glycobiology Advance Access originally published online on September 30, 2009
Glycobiology 2010 20(1):107-117; doi:10.1093/glycob/cwp153
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© The Author 2009. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Lessons from GNE-deficient embryonic stem cells: sialic acid biosynthesis is involved in proliferation and gene expression

Wenke Weidemann1,2, Christian Klukas3, Andreas Klein4, Andreas Simm5, Falk Schreiber3,6 and Rüdiger Horstkorte2

2 Institute of Physiological Chemistry, Martin Luther University Halle-Wittenberg, Hollystr. 1, D-06114 Halle
3 Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstr. 3, 06466 Gatersleben
4 Institute of Molecular Biology and Bioinformatics, Charité, Campus Benjamin Franklin, Arnimalle 22, 14195 Berlin
5 Department of Cardio-Thoracic Surgery, Ernst-Grube-Str. 40, D-06120 Halle
6 Institute for Computer Science, Martin Luther University Halle-Wittenberg, Von-Seckendorff-Platz 1, D-06120 Halle, Germany

1 To whom correspondence should be addressed: Tel: +49-345-5573806; Fax: +49-345-5573807; e-mail: wenke.weidemann{at}medizin.uni-halle.de

Received on March 17, 2009; revised on September 21, 2009; accepted on September 24, 2009

Sialic acids are widely expressed as terminal carbohydrates on glycoconjugates of eukaryotic cells. They are involved in a variety of cellular functions, such as cell adhesion or signal recognition. The key enzyme of sialic acid biosynthesis is the bifunctional UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (GNE), which catalyzes the first two steps of sialic acid biosynthesis in the cytosol. Inactivation of GNE causes early embryonic lethality. In this study, we analyzed wild-type and GNE-deficient embryonic stem cells from mice. We found for the first time that proliferation is directly correlated with GNE-expression and the cellular sialic acid concentration. Furthermore, we identified growth-related genes that are differentially expressed in GNE-deficient embryonic stem cells compared to wild-type embryonic stem cells.

Key words: cell differentiation / N-acetylmannosamine / N-acetylmannosamine kinase / UDP-N-acetylglucosamine-2- epimerase


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