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Glycobiology Advance Access originally published online on June 19, 2009
Glycobiology 2009 19(9):950-957; doi:10.1093/glycob/cwp089
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© The Author 2009. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Communication

The specific localization of seminolipid molecular species on mouse testis during testicular maturation revealed by imaging mass spectrometry

Naoko Goto-Inoue1,2, Takahiro Hayasaka2, Nobuhiro Zaima2 and Mitsutoshi Setou2

2 Department of Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan

1 To whom correspondence should be addressed: Tel: +81-53-435-2085; Fax: +81-53-435-2292; e-mail: naoko.goto.inoue{at}gmail.com

Received on April 20, 2009; revised on June 14, 2009; accepted on June 15, 2009

More than 90% of the glycolipid in mammalian testis consists of a unique sulfated glyceroglycolipid called seminolipid. The galactosylation of the molecule is catalyzed by UDP-galactose:ceramide galactosyltransferase (CGT). Disruption of the CGT gene in mice results in male infertility due to the arrest of spermatogenesis, indicating that seminolipid plays an important role in reproductive function. Seminolipid molecules can be assigned to different molecular species based on the fatty acid composition. In this report, we investigated the localizations of the molecular species of seminolipid by imaging mass spectrometry and demonstrated that major molecule (C16:0-alkyl-C16:0-acyl) was expressed throughout the tubules: some (C16:0-alkyl-C14:0-acyl and C14:0-alkyl-C16:0-acyl) were predominantly expressed in spermatocytes and the other (C17:0-alkyl-C16:0-acyl) was specifically expressed in spermatids and spermatozoa. This is the first report to show the cell-specific localization of each molecular species of seminolipid during testicular maturation.

Key words: imaging / MALDI / mass spectrometry / seminolipid / testis


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