Glycobiology, Vol 9, 947-955, Copyright © 1999 by Oxford University Press
QW Fan, K Uchimura, Y Yuzawa, S Matsuo, C Mitsuoka, R Kannagi, H Muramatsu, K Kadomatsu and T Muramatsu
GlcNAc-6- O -sulfotransferase is involved in formation of 6-sulfo- N -
acetyllactosamine-containing structures such as 6-sulfo sialyl Lewis x. We
investigated the mode of expression of GlcNAc-6-O-sulfotransferase during
postimplantation embryogenesis in the mouse by in situ hybridization.
Sulfotransferase mRNA was not detected on embryonic day (E) 6.5, while on
E7.5 it was detected in the mesoderm, ectoderm, and ectoplacental cone. On
E10.5, the sulfotransferase signals were mainly observed in the nervous
tissue. On E12.5 and 13.5, various tissues in the process of
differentiation expressed this mRNA. Several epithelial and mesenchymal
tissues undergoing epithelial-mesenchymal interactions strongly expressed
the mRNA. For example, in the developing tooth strong sulfotransferase mRNA
expression was found only in the condensing mesenchyme on E13.5. On E13.5
and 15.5, the sites showing intense expression of the sulfotransferase
again became restricted. In the brain, sulfotransferase mRNA was frequently
found as discrete signals in narrow regions. These results suggest that
6-sulfo- N - acetyllactosamine structures have important roles in
development. On E13.5 and 15.5, G152 (6-sulfo sialyl Lewis x antigen) was
expressed in the neocortex, and AG223 (6-sulfo Lewis x antigen) in the
thalamus and neocortex where the sulfotransferase signal was detected.
However, in other organs, expression of these antigens did not correlate
with the sulfotransferase mRNA, implicating complex nature of regulation of
expression of the fucosyl 6-sulfo antigens.
ORIGINAL ARTICLES
Spatially and temporally regulated expression of N-acetylglucosamine-6- O-sulfotransferase during mouse embryogenesis
Department of Biochemistry and the 3rd Department of Internal Medicine, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan and the Program of Experimental Pathology, Aichi Cancer Center, Research Insti.
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