Glycobiology, Vol 9, 927-933, Copyright © 1999 by Oxford University Press
AC Vilela-Silva, AP Alves, AP Valente, VD Vacquier and PA Mourao
The egg jelly coats of sea urchins contains sulfated polysaccharides
responsible for inducing the sperm acrosome reaction which is an obligatory
event for sperm binding to, and fusion with, the egg. Here, we extend our
study to the sea urchin Strongylocentrotus franciscanus. The egg jelly of
this species contains a homofucan composed of 2- O - sulfated, 3-linked
units which is the simplest structure ever reported for a sulfated fucan.
This polysaccharide was compared with other sulfated alpha-l-fucans as
inducers of acrosome reaction in conspecific and heterospecific sperm.
Although all these fucans are linear polymers composed of 3-linked
alpha-l-fucopyranosyl units, they differ in the proportions of 2- O - and
4- O -sulfation. The reactivity of the sperm of each species is more
sensitive to the egg jelly sulfated fucan found in their own species. The
reactivity of the sperm does not correlate with the charge density of the
fucan, but with the proportion of 2- O - and 4- O - sulfation. The pattern
of sulfation may be an important feature for recognition of fucans by the
sperm receptor contributing to the species-specificity of fertilization.
Structure of the sulfated alpha-L-fucan from the egg jelly coat of the sea urchin strongylocentrotus franciscanus: patterns of preferential 2- O- and 4-O-sulfation determine sperm cell recognition [In Process Citation]
Laboratorio de Tecido Conjuntivo, Hospital Universitario and Departamento de Bioquimica Medica, Centro de Ciencias da Saude, Universidade Federal do Rio de Janeiro, Caixa Postal 68041, Rio de Janeiro, RJ, 21941-590, Brazil, Centro Naciona.
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