Glycobiology, Vol 9, 311-321, Copyright © 1999 by Oxford University Press
M Davril, S Degroote, P Humbert, C Galabert, V Dumur, JJ Lafitte, G Lamblin and P Roussel
Bronchial mucins were purified from the sputum of 14 patients suffering
from cystic fibrosis and 24 patients suffering from chronic bronchitis,
using two CsBr density-gradient centrifugations. The presence of DNA in
each secretion was used as an index to estimate the severity of infection
and allowed to subdivide the mucins into four groups corresponding to
infected or noninfected patients with cystic fibrosis, and to infected or
noninfected patients with chronic bronchitis. All infected patients
suffering from cystic fibrosis were colonized by Pseudomonas aeruginosa. As
already observed, the mucins from the patients with cystic fibrosis had a
higher sulfate content than the mucins from the patients with chronic
bronchitis. However, there was a striking increase in the sialic acid
content of the mucins secreted by severely infected patients as compared to
noninfected patients. Thirty- six bronchial mucins out of 38 contained the
sialyl-Lewis x epitope which was even expressed by subjects phenotyped as
Lewis negative, indicating that at least one alpha1,3 fucosyltransferase
different from the Lewis enzyme was involved in the biosynthesis of this
epitope. Finally, the sialyl-Lewis x determinant was also overexpressed in
the mucins from severely infected patients. Altogether these differences in
the glycosylation process of mucins from infected and noninfected patients
suggest that bacterial infection influences the expression of
sialyltransferases and alpha1,3 fucosyltransferases in the human bronchial
mucosa.
ORIGINAL ARTICLES
The sialylation of bronchial mucins secreted by patients suffering from cystic fibrosis or from chronic bronchitis is related to the severity of airway infection
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