Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (12)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Whitham, K. M.
Right arrow Articles by Brown, G. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Whitham, K. M.
Right arrow Articles by Brown, G. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Glycobiology, Vol 9, 285-291, Copyright © 1999 by Oxford University Press

ORIGINAL ARTICLES

An improved method for the structural profiling of keratan sulfates: analysis of keratan sulfates from brain and ovarian tumors

KM Whitham, JL Hadley, HG Morris, SM Andrew, IA Nieduszynski and GM Brown
Department of Biological Sciences, Institute of Environmental and Natural Sciences, Lancaster University, Bailrigg, Lancaster LA1 4YQ, UK.

A previously developed method for the structural fingerprinting of keratan sulfates (Brown et al., Glycobiology, 5, 311-317, 1995) has been adapted for use with oligosaccharides fluorescently labeled with 2- aminobenzoic acid following keratanase II digestion. The oligosaccharides are separated by high-pH anion-exchange chromatography on a Dionex AS4A-SC column. This methodology permits quantitative analysis of labeled oligosaccharides which can be detected at the sub- nanogram ( approximately 100 fmol) level. Satisfactory calibration of this method can be achieved using commercial keratan sulfate standards. Keratan sulfates from porcine brain phosphocan and human ovarian tumors have been examined using this methodology, and their structural features are discussed.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 GlycobiologyHome page
N. T. Seyfried, C. D. Blundell, A. J. Day, and A. Almond
Preparation and application of biologically active fluorescent hyaluronan oligosaccharides
Glycobiology, March 1, 2005; 15(3): 303 - 312.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
G. Magro, D. Perissinotto, M. Schiappacassi, S. Goletz, A. Otto, E.-C. Muller, M. Bisceglia, G. Brown, T. Ellis, S. Grasso, et al.
Proteomic and Postproteomic Characterization of Keratan Sulfate-Glycanated Isoforms of Thyroglobulin and Transferrin Uniquely Elaborated by Papillary Thyroid Carcinomas
Am. J. Pathol., July 1, 2003; 163(1): 183 - 196.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
A. H.K. Plaas, L. A. West, and R. J. Midura
Keratan sulfate disaccharide composition determined by FACE analysis of keratanase II and endo-{beta}-galactosidase digestion products
Glycobiology, October 1, 2001; 11(10): 779 - 790.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
R. M. Lauder, T. N. Huckerby, and I. A. Nieduszynski
A fingerprinting method for chondroitin/dermatan sulfate and hyaluronan oligosaccharides
Glycobiology, April 1, 2000; 10(4): 393 - 401.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.