Glycobiology, Vol 9, 1-12, Copyright © 1999 by Oxford University Press
M Nakamura, Y Furukawa, R Sasaki, J Masuyama, J Kikuchi, S Iwase, T Kudo, H Narimatsu, S Asakura, S Fujiwara and J Inokuchi
Expression mechanism of CD15s (sialyl-Lex, sLex) antigen has been
investigated using human B lymphoid cell lines. sLexstructures were not
expressed in mature B lymphoids but highly expressed in pre-B leukemia and
pre-B lymphoma cell lines. The expression site was mainly on the O - linked
oligosaccharide chains and E-selectin mediated-cell adhesion capability of
sLex-positive cells were significantly suppressed by benzyl-alpha-GalNAc
treatment. Subsequently, the bases of the sLexexpression control mechanism
were examined at the levels of enzymatic activities and transcripts of
glycosyltransferases. (1) The activities of
alpha1-->3fucosyltransferase, alpha2-- >3sialyltransferase,
beta1-->4Gal-transferase, and elongation beta1--
>3GlcNAc-transferase, did not correlate with sLexexpression levels. (2)
The transcripts of Fuc-TVII were not parallel with sLexexpression, and
those of ST3Gal IV and beta1-->4Gal-transferase were constitutively
detected in all cell lines tested. (3) There was no detectable enzyme
activity for core 3 and 4 backbone structure synthesis in human B cell
lines. (4) By contrast, the enzyme activities and transcripts of UDP-
GlcNAc:Galbeta1-->3GalNAc (GlcNAc to GalNAc) beta1-->6 N -
acetylglucosaminyltransferase (Core2GnT) had significant correlation with
the cell surface expression of sLexantigen. (5) Moreover, Western blot
analysis revealed the presence of a major approximately 150 kDa
glycoprotein that carries O -linked oligosaccharides recognized by anti-
sLexmonoclonal antibody in sLex-positive pre-B leukemia cell lines. This
correlation of Core2GnT with CD15s expression suggests that Core2GnT is a
regulator of the cell surface expression of sLexin human pre-B lymphoid
cells.
ORIGINAL ARTICLES
UDP-GlcNAc:Galbeta1-->3GalNAc (GlcNAc to GalNAc) beta1-->6N- acetylglucosaminyltransferase holds a key role on the control of CD15s expression in human pre-B lymphoid cell lines
Division of Hemopoiesis and Division of Hemostasis and Thrombosis Research, Institute of Hematology, and Department of Allergy and Collagen Disease, Jichi Medical School, Minamikawachi, Tochigi 329-04, Japan.
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