Glycobiology, Vol 8, 731-740, Copyright © 1998 by Society for Glycobiology
IE VanderElst and A Datti
The distribution of the Golgi enzyme beta1, 6-N-
acetylglucosaminyltransferase (core 2 GlcNAc-T for short) has been
investigated in several tissue and cell systems by combining the potentials
of a polyclonal antibody and a novel, sensitive fluorescent enzyme assay.
In normal rat tissues, levels of the protein were found to vary and as a
general trend did not correlate with enzyme activities. Additionally, we
observed tissue-specific core 2 GlcNAc-T forms of various size: 75 kDa
(liver), 70 kDa (spleen), 60 kDA (heart), and 50 kDa (heart and lung).
These forms might arise from differential protein modifications;
alternatively, the smaller form may be a product of proteolytic cleavage,
given the presence of a catalytically inactive 50 kDa species in rat serum.
Chinese hamster ovary (CHO), MDAY-D2, PSA- 5E, and PYS-2 cell lines
consistently displayed a 70 kDa enzyme. When induced to retrodifferentiate
in the presence of butyrate + cholera toxin, CHO cells exhibited a 21-fold
increase in enzyme activity, while protein levels remained constant. A
similar trend was observed in the embryonal endoderm cell lines PSA-5E and
PYS-2, where an approximately 100-fold difference in core 2 GlcNAc-T
activity was found notwithstanding unchanged amounts of the protein and
identical mRNA levels, as evidenced by RT-PCR. In contrast, levels of core
2 GlcNAc-T activity in MDAY-D2 cells correlated well with protein
expression. Taken together, these observations demonstrate that core 2
GlcNAc-T expression may be subjected to multiple mechanisms of regulation
and suggest that in at least some instances (i.e., PSA-5E and PYS-2 cells)
expression may be regulated exclusively via posttranslational mechanism(s)
of control.
ORIGINAL ARTICLES
beta1,6 N-acetylglucosaminyltransferase (core 2 GlcNAc-T) expression in normal rat tissues and different cell lines: evidence for complex mechanisms of regulation
Department of Cell and Molecular Biology, Section of Biochemistry and Molecular Biology, University of Perugia, 06126 Perugia, Italy.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Tan and P.-W. Cheng Mucin Biosynthesis: Identification of the cis-Regulatory Elements of Human C2GnT-M Gene Am. J. Respir. Cell Mol. Biol., June 1, 2007; 36(6): 737 - 745. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. M. Ben-Mahmud, G. E. Mann, A. Datti, A. Orlacchio, E. M. Kohner, and R. Chibber Tumor Necrosis Factor-{alpha} in Diabetic Plasma Increases the Activity of Core 2 GlcNAc-T and Adherence of Human Leukocytes to Retinal Endothelial Cells: Significance of Core 2 GlcNAc-T in Diabetic Retinopathy Diabetes, November 1, 2004; 53(11): 2968 - 2976. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T.-Y. Yen, B. A. Macher, S. Bryson, X. Chang, I. Tvaroska, R. Tse, S. Takeshita, A. M. Lew, and A. Datti Highly Conserved Cysteines of Mouse Core 2 {beta}1,6-N-Acetylglucosaminyltransferase I Form a Network of Disulfide Bonds and Include a Thiol That Affects Enzyme Activity J. Biol. Chem., November 14, 2003; 278(46): 45864 - 45881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Chibber, B. M. Ben-Mahmud, G. E. Mann, J. J. Zhang, and E. M. Kohner Protein Kinase C {beta}2-Dependent Phosphorylation of Core 2 GlcNAc-T Promotes Leukocyte-Endothelial Cell Adhesion: A Mechanism Underlying Capillary Occlusion in Diabetic Retinopathy Diabetes, June 1, 2003; 52(6): 1519 - 1527. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. R. Falkenberg, K. Alvarez, C. Roman, and N. Fregien Multiple transcription initiation and alternative splicing in the 5' untranslated region of the core 2 {beta}1-6 N-acetylglucosaminyltransferase I gene Glycobiology, June 1, 2003; 13(6): 411 - 418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Schwientek, J.-C. Yeh, S. B. Levery, B. Keck, G. Merkx, A. G. van Kessel, M. Fukuda, and H. Clausen Control of O-Glycan Branch Formation. MOLECULAR CLONING AND CHARACTERIZATION OF A NOVEL THYMUS-ASSOCIATED CORE 2 beta 1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE J. Biol. Chem., April 6, 2000; 275(15): 11106 - 11113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Schwientek, M. Nomoto, S. B. Levery, G. Merkx, A. G. van Kessel, E. P. Bennett, M. A. Hollingsworth, and H. Clausen Control of O-Glycan Branch Formation. MOLECULAR CLONING OF HUMAN cDNA ENCODING A NOVEL beta 1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE FORMING CORE 2 AND CORE 4 J. Biol. Chem., February 19, 1999; 274(8): 4504 - 4512. [Abstract] [Full Text] [PDF]
|
|