Glycobiology, Vol 8, 605-613, Copyright © 1998 by Society for Glycobiology
E Mas, E Pasqualini, N Caillol, A El Battari, C Crotte, D Lombardo and MO Sadoulet
Human pancreatic cancer is characterized by an alteration in fucose-
containing surface blood group antigens such as H antigen, Lewis b, Lewis
y, and sialyl-Lewis. These carbohydrate determinants can be synthesized by
sequential action of alpha(2,3) sialyltransferases or alpha(1,2)
fucosyltransferases (Fuc-T) and alpha(1,3/1,4) fucosyltransferases on
(poly)N-acetyllactosamine chains. Therefore, the expression and the
function of seven fucosyltransferases were investigated in normal and
cancer pancreatic tissues and in four pancreatic carcinoma cell lines.
Transcripts of FUT1, FUT2, FUT3, FUT4, FUT5, and FUT7 were detected by
RT-PCR in carcinoma cell lines as well as in normal and tumoral tissues.
Interestingly, the FUT6 message was only detected in tumoral tissues.
Analysis of the acceptor substrate specificity for fucosyltransferases
indicated that alpha(1,2) Fuc-T, alpha(1,3) Fuc-T, and alpha(1,4) Fuc-T
were expressed in microsome preparations of all tissues as demonstrated by
fucose incorporation into phenyl beta-d-galactoside, 2'-fucosyllactose,
N-acetyllactosamine, 3'-sialyl-N-acetyllactosamine, and lacto-N-biose.
However, these fucosyltransferase activities varied between tissues. A
substantial decrease of alpha(1,2) Fuc-T activity was observed in tumoral
tissues and cell lines compared to normal tissues. Conversely, the activity
of alpha(1,4) Fuc-T, which generates Lewis a and sialyl-Lewis a structures,
and that of alpha(1,3) Fuc-T, able to generate a lactodifucotetraose
structure, were very important in SOJ-6 and BxPC-3 cell lines. These
increases correlated with an enhanced expression of Lewis a, sialyl-Lewis
a, and Lewis y on the cell surface. The activity of alpha(1,3) Fuc-T, which
participates in the synthesis of the sialyl- Lewis x structure, was not
significantly modified in cell lines compared to normal tissues. However,
the sialyl-Lewis x antigen was expressed preferentially on the surface of
SOJ-6 and BxPC-3 cell lines but was not detected on Panc-1 and MiaPaca-2
cell lines suggesting that several alpha(1,3) Fuc-T might be involved in
sialyl-Lewis x synthesis.
ORIGINAL ARTICLES
Fucosyltransferase activities in human pancreatic tissue: comparative study between cancer tissues and established tumoral cell lines
INSERM-U. 260, Unite de Recherche de Physiopathologie des Regulations Hormono-Nutritionnelles, Faculte de Medecine, 27 Boulevard Jean Moulin, 13385 Marseille-Cedex 5, France.
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