Glycobiology, Vol 8, 463-471, Copyright © 1998 by Society for Glycobiology
YG Brickman, V Nurcombe, MD Ford, JT Gallagher, PF Bartlett and JE Turnbull
Heparan sulfate (HS) glycosaminoglycans are essential modulators of
fibroblast growth factor (FGF) activity both in vivo and in vitro, and
appear to act by cross-linking particular forms of FGF to appropriate FGF
receptors. We have recently isolated and characterized two separate HS
pools derived from immortalized embryonic day 10 mouse neuroepithelial 2.3D
cells: one from cells in log growth phase, which greatly potentiates the
activity of FGF-2, and the other from cells undergoing contact-inhibition
and differentiation, which preferentially activates FGF-1. These two pools
of HS have very similar functional activities to those species isolated
from primary neuroepithelial cells at corresponding stages of active
proliferation or differentiation. We present here a structural comparison
between these cell line HS species to establish the nature of the changes
that occur in the biosynthesis of HS. A combination of chemical and
enzymatic cleavage, low pressure chromatography and strong anion-exchange
HPLC were used to generate full chain models of each species. Overall, the
HS pools synthesized in the dividing cell line pools possessed less complex
sulfation than those derived from more differentiated, growth arrested
cells.
ORIGINAL ARTICLES
Structural comparison of fibroblast growth factor-specific heparan sulfates derived from a growing or differentiating neuroepithelial cell line
Department of Anatomy and Cell Biology, University of Melbourne, Victoria, Australia.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. E. Guimond, T. M. Puvirajesinghe, M. A. Skidmore, I. Kalus, T. Dierks, E. A. Yates, and J. E. Turnbull Rapid Purification and High Sensitivity Analysis of Heparan Sulfate from Cells and Tissues: TOWARD GLYCOMICS PROFILING J. Biol. Chem., September 18, 2009; 284(38): 25714 - 25722. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zako, J. Dong, O. Goldberger, M. Bernfield, J. T. Gallagher, and J. A. Deakin Syndecan-1 and -4 Synthesized Simultaneously by Mouse Mammary Gland Epithelial Cells Bear Heparan Sulfate Chains That Are Apparently Structurally Indistinguishable J. Biol. Chem., April 4, 2003; 278(15): 13561 - 13569. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Thomas, A. Clayton, J. Thomas, M. Davies, and R. Steadman Structural and Functional Changes in Heparan Sulfate Proteoglycan Expression Associated with the Myofibroblastic Phenotype Am. J. Pathol., March 1, 2003; 162(3): 977 - 989. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ford-Perriss, S. E. Guimond, U. Greferath, M. Kita, K. Grobe, H. Habuchi, K. Kimata, J. D. Esko, M. Murphy, and J. E. Turnbull Variant heparan sulfates synthesized in developing mouse brain differentially regulate FGF signaling Glycobiology, November 1, 2002; 12(11): 721 - 727. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Okada, S. Yamada, M. Toyoshima, J. Dong, M. Nakajima, and K. Sugahara Structural Recognition by Recombinant Human Heparanase That Plays Critical Roles in Tumor Metastasis. HIERARCHICAL SULFATE GROUPS WITH DIFFERENTIAL EFFECTS AND THE ESSENTIAL TARGET DISULFATED TRISACCHARIDE SEQUENCE J. Biol. Chem., November 1, 2002; 277(45): 42488 - 42495. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Powell, D. G. Fernig, and J. E. Turnbull Fibroblast Growth Factor Receptors 1 and 2 Interact Differently with Heparin/Heparan Sulfate. IMPLICATIONS FOR DYNAMIC ASSEMBLY OF A TERNARY SIGNALING COMPLEX J. Biol. Chem., August 2, 2002; 277(32): 28554 - 28563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Bame Heparanases: endoglycosidases that degrade heparan sulfate proteoglycans Glycobiology, June 1, 2001; 11(6): 91R - 98R. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Pye, R. R. Vives, P. Hyde, and J. T. Gallagher Regulation of FGF-1 mitogenic activity by heparan sulfate oligosaccharides is dependent on specific structural features: differential requirements for the modulation of FGF-1 and FGF-2 Glycobiology, November 1, 2000; 10(11): 1183 - 1192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Bame, I. Venkatesan, H. D. Stelling, and S. Tumova The spacing of S-domains on HS glycosaminoglycans determines whether the chain is a substrate for intracellular heparanases Glycobiology, July 1, 2000; 10(7): 715 - 726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Nurcombe, C. E. Smart, H. Chipperfield, S. M. Cool, B. Boilly, and H. Hondermarck The Proliferative and Migratory Activities of Breast Cancer Cells Can Be Differentially Regulated by Heparan Sulfates J. Biol. Chem., September 22, 2000; 275(39): 30009 - 30018. [Abstract] [Full Text] [PDF]
|
|