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Glycobiology, Vol 8, 425-432, Copyright © 1998 by Society for Glycobiology

ORIGINAL ARTICLES

Production and characterization of a monoclonal antibody able to discriminate galectin-1 from galectin-2 and galectin-3

JD Cornillot, M Pontet, C Dupuy, A Chadli, M Caron, R Joubert-Caron, P Bourin and D Bladier
Laboratoire de Biochimie et Technologie des Proteines (EA 1625), Universite Paris Nord, UFR Sante, Medecine et Biologie Humaine, Bobigny, France.

Antisera raised against galectin-1 exhibit crossreactivities with other galectins or related molecules. In order to overcome this problem, a monoclonal antibody to human brain galectin-1 was obtained by selecting clones without reactivity toward galectin-3. This mAb specifically bound galectin-1 of various animal origins but neither galectin-2 nor galectin-3. Western-blotting analysis of soluble human brain extracts after 2D gel electrophoresis revealed only the two most acidic isoforms of galectin-1. The ability of this mAb to bind galectin-1/asialofetuin complexes indicates that its epitope is not localized in the carbohydrate recognition domain of galectin-1. This particularity induces with efficiency its monospecificity.
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