Glycobiology vol 7 no 1 pp. 137-146, 1997
© 1997
research-article |
Unique tissue distribution of a mouse macrophage C-type lectin
Department of Cancer Biology and Molecular Immunology (formerly Division of Chemical Toxicology and Immunochemistiy), Faculty of Pharmaceutical Sciences, The University of Tokyo Hongo, Tokyo 113, Japan
1Department of Anatomy, Kyorin University School of Medicine Mitaka, Tokyo 181, Japan
2To whom correspondence should be addressed at: Department of Cancer Biology and Molecular Immunology, Faculty of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113. Japan
Received on July 25, 1996; revised on August 19, 1996; accepted on August 25, 1996
We examined mouse tissue for the expression of macrophage galactose/N-acetylgalactosamine-specific C-type lectin using a rat monoclonal antibody (mAb) specific for this lectin (mAb LOM-14). The binding of mAb LOM-14 was detected in detergent extracts from tissue by means of immunoblotting analysis. It was shown that this mAb did not cross-react with mouse hepatic lectins, a structural homologue. The macrophage lectin was widely distributed among various mouse tissues as judged by the affinity isolation followed by the immunochemical detection. The exceptions were brain, liver, kidney, small intestine, and peripheral blood. Extracts from these organs exhibited, at best, very weak signals upon mAb LOM-14 binding, despite the presence of cells expressing macrophage markers. The most intense signal was observed in the extract from skin, suggesting that cells expressing this lectin are abundant in skin. The tissues shown to contain this lectin were further investigated by immunohistochemical staining of the sections. Cells were distributed in the connective tissue and in the interstice, particularly the dermis and subcutaneous layer of skin. Cells localized in the epithelium of skin (epidermis) or other epithelia that we examined were not stained. Perivascular localization of cells stained with mAb LOM-14 was also demonstrated in cardiac and skeletal muscle tissues. Immunoelectron microscopy revealed the presence of this lectin along the rough endoplasmic reticulum. In conclusion, the distribution of C-type lectin specific for galactose/N-acetylgalactosamine in mice was unique. The connective tissue-specific distribution should provide important information on the biological role of this lectin.
lectin macrophage calcium-type lectin connective tissue
3Present address: Department of Hygienic Chemistry, Toho University School of Pharmaceutical Science, Funabashi, Chiba 274, Japan
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