Glycobiology vol 7 no 1 pp. 129-136, 1997
© 1997
research-article |
Modification of CD43 and other lymphocyte O-glycoproteins by core 2 N-acetylglucosaminyltransferase
The Biomedical Research Centre, University of British Columbia Vancouver, V6T 1Z3 British Columbia, Canada
1The British Columbia Institute of Technology Burnaby, V5G 3H2 British Columbia, Canada
2The Samuel Lunenfeld Research Institute Toronto, M5G IXS Ontario, Canada
3The Department of Pathology and Laboratory Medicine, University of British Columbia Vancouver, British Columbia, Canada
4To whom correspondence should be addressed at: Biomedical Research Centre, 2222 Health Sciences Mall, University of British Columbia, Vancouver, V6T 1Z3 British Columbia, Canada
Received on July 12, 1996; revised on August 20, 1996; accepted on August 23, 1996
CD43, the major leukocyte sialoglycoprotein, is expressed on T lymphocytes in two predominant glycoforms. CD43 115 kDa is a pan T cell marker and is specifically recognized by the monoclonal antibody S7. CD43 130 kDa is associated with T cell activation and is specifically recognized by the monoclonal antibody 1B11. The thymoma EL-4 has been identified to express mainly CD43 115 kDa and little or no CD43 130 kDa. Transfection of EL-4 cells with core 2 ß1
6N-acetylgIucosaminyltransferase (C2GnT), an enzyme in the O-glycan biosynthesis pathway, resulted in an enhanced expression of the 1B11 epitope, CD43 130 kDa, and a loss of expression of the S7 epitope, CD43 115 kDa. Analysis of CD43 by SDS-PAGE revealed that CD43 in C2GnT transfected EL-4 cells has a molecular weight of 125 kDa compared to 115 kDa in nontransfected or control transfected EL-4 cells. SDS-PAGE analysis of three other lymphocyte O-glycoproteins, CD44, CD45, and RPTPa., revealed that C2GnT expression resulted in a molecular weight increase of approximately 3–5 kDa for each of these three cell surface glycoproteins. Our data indicate that, while CD43 may be a predominant substrate for C2GnT, other lymphocyte O-glycoproteins are also modified by this glycosyltransferase. Increased reactivity of cells with the monoclonal antibody 1B11, which specifically detects the expression of murine CD43 130 kDa, may thus be a marker of increases in branching of O-linked glycans generally.
CD43 core 2 N-acetylglucosaminyltransferase lymphocyte glycoproteins
5Permanent address: The British Columbia Institute of Technology, 3700 Willingdon Ave. Burnaby, V5G 3H2 British Columbia, Canada
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