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Glycobiology vol 5 no 8 pp. 791-796, 1995
© 1995

research-article

Glycan structure of a heptose-containing S-layer glycoprotein of Bacillus thermoaerophilus

Paul Kosma1, Thomas Wugeditsch, Rudolf Christian2, Sonja Zayni and Paul Messner3

Zentrum für Ultrastukturforschung und Ludwig Boltzmann-Institut für Molekulare Nanotechnologie
1Institut für Chemie, Universität für Bodenkulter Gregor-Mendel-Stra$$$e 33, A-1180 Wien
2Scientific Software Comp. A-1140 Wien, Austria

3To whom correspondence should be addressed

Received on June 14, 1995; revised on July 27, 1995; accepted on July 31, 1995

The characterization of the S-layer glycoprotein of Bacillus ihermoaerophilus revealed unexpected novelties. The isolation and purification procedure had to be changed due to complete solubility in aqueous buffers of the constituting S-layer protomers. Upon degradation of the S-layer glycoprotein by pronase and purification of the products by gel filtration, ion-exchange chromatography, chromatofocusing and HPLC, one representative glycopeptide fraction was selected for further characterization. From the combined evidence of composition analysis, chemical degradation, NMR spectroscopy experiments and comparison with synthesized model substance, we propose the following repeating unit structure of the glycan chain:

->4)-{alpha}-L-Hhap-(1->3)-ß-D-glycero-D-manno-Hepp-(1->

This is the first description of heptose residues occurring as a constituent of S-layer glycoproteins of gram-positive eubacteria.

eubacteria glycoprotein heptose structure determination surface layer (S-layer)


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