Glycobiology vol 2 no 4 pp. 299-312, 1992
© 1992
research-article |
Carbohydrate structure of Marburg virus glycoprotein
1Biochemisches Institut am Klinikum der Universität Friedrichstraße 24, D-6300 Giessen
2Institut für Virologie, Philipps-Universität Robert-Koch-Straße 17, D-3550 Marburg, FRG
3To whom correspondence should be addressed
Received on March 18, 1992; accepted on April 23, 1992
Marburg virus was propagated in E6 cells, a cloned cell line of Vero cells, in the presence of [6-3H]glucosamine Radiolabelled viral glycoprotein was digested with trypsin, and oligosaccharides were liberated by sequential treatment with endo-ß-N-acetylglucosaminidase H, peptide-N4-(N-acetyl-ß-glucosaminyl)asparagine amidase F and O-glycosidase, by ß-elimination, and by alkaline hydrolysis. After fractionation by HPLC and gel filtration, glycans were characterized chromatographically, by digestion with exoglycosidases and, in part, by methylation analysis and liquid secondary ion mass spectrometry. The oligosaccharide structures thus established include oligomannosidic and hybrid-type N-glycans, as well as neutral fucosylated bitri- and tetraantennary species, most of which carry an additional bisecting N-acetylglucosamine. In addition, high amounts of neutral mucin-type O-glycans with type-1 and type-2 core structures were detected. None of the glycans present in this viral glycoprotein carried sialic acid residues.
Filoviridae glycoprotein Marburg virus oligosaccharides structure analysis
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  E. Mittler, L. Kolesnikova, T. Strecker, W. Garten, and S. Becker Role of the Transmembrane Domain of Marburg Virus Surface Protein GP in Assembly of the Viral Envelope J. Virol., April 15, 2007; 81(8): 3942 - 3948. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Ji, G. G. Olinger, S. Aris, Y. Chen, H. Gewurz, and G. T. Spear Mannose-binding lectin binds to Ebola and Marburg envelope glycoproteins, resulting in blocking of virus interaction with DC-SIGN and complement-mediated virus neutralization J. Gen. Virol., September 1, 2005; 86(9): 2535 - 2542. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Kolesnikova, B. Berghofer, S. Bamberg, and S. Becker Multivesicular Bodies as a Platform for Formation of the Marburg Virus Envelope J. Virol., November 15, 2004; 78(22): 12277 - 12287. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Takada, K. Fujioka, M. Tsuiji, A. Morikawa, N. Higashi, H. Ebihara, D. Kobasa, H. Feldmann, T. Irimura, and Y. Kawaoka Human Macrophage C-Type Lectin Specific for Galactose and N-Acetylgalactosamine Promotes Filovirus Entry J. Virol., March 15, 2004; 78(6): 2943 - 2947. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Feldmann, V. E. Volchkov, V. A. Volchkova, U. Stroher, and H.-D. Klenk Biosynthesis and role of filoviral glycoproteins J. Gen. Virol., December 1, 2001; 82(12): 2839 - 2848. [Full Text] [PDF]
|
|
|
|
 |
|
 S. Liedtke, H. Geyer, M. Wuhrer, R. Geyer, G. Frank, R. Gerardy-Schahn, U. Zahringer, and M. Schachner Characterization of N-glycans from mouse brain neural cell adhesion molecule Glycobiology, May 1, 2001; 11(5): 373 - 384. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sänger, E. Mühlberger, E. Ryabchikova, L. Kolesnikova, H.-D. Klenk, and S. Becker Sorting of Marburg Virus Surface Protein and Virus Release Take Place at Opposite Surfaces of Infected Polarized Epithelial Cells J. Virol., February 1, 2001; 75(3): 1274 - 1283. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Schmitt, D. Glebe, K. Alving, T. K. Tolle, M. Linder, H. Geyer, D. Linder, J. Peter-Katalinic, W. H. Gerlich, and R. Geyer Analysis of the Pre-S2 N- and O-Linked Glycans of the M Surface Protein from Human Hepatitis B Virus J. Biol. Chem., April 23, 1999; 274(17): 11945 - 11957. [Abstract] [Full Text] [PDF]
|
|