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Glycobiology vol 2 no 4 pp. 293-298, 1992
© 1992

research-article

Reducing the overlap problem in the proton NMR spectra of oligosaccharides by application of pseudo-four-dimensional homonuclear HOHAHA-HOHAHA-COSY

T.J. Rutherford and S.W. Homans

Department of Biochemistiy, University of Dundee Dundee DD1 4HN, UK

Received on February 28, 1992; accepted on April 24, 1992

A new homonuclear NMR experiment is described for the assignment of the proton NMR spectra of oligosaccharides, namely HOHAHA-HOHAHA-COSY (where HOHAHA is homonuclear Hartmann-Hahn spectroscopy and COSY is correlated spectroscopy). While this technique may formally be thought of as a four-dimensional NMR experiment, by use of selective pulses it is demonstrated that the analogous pseudo-four-dimensional experiment is a valuable improvement over conventional three-dimensional HOHAHA-COSY, in that the degree of resonance overlap is markedly reduced by the dispersion of resonances into a fourth effective dimension. The technique is demonstrated by application to the biantennary nonasaccharide Galß1-4-GlcNAcß1-2Man{alpha}1-6(Galß1-4GlcNAcß1-2Man{alpha}1-3)Manß1-4GlcNacß1-4GlcNac

four-dimensional NMR N-linked glycan serotransferrin


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