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Glycobiology Advance Access originally published online on February 24, 2009
Glycobiology 2009 19(6):624-632; doi:10.1093/glycob/cwp029
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© The Author 2009. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Class IIC {alpha}-mannosidase AfAms1 is required for morphogenesis and cellular function in Aspergillus fumigatus

Yanjie Li, Wenxia Fang, Lei Zhang, Haomiao Ouyang, Hui Zhou, Yuanming Luo and Cheng Jin1

Key Laboratory of Systematic Mycology and Lichenology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China

1 To whom correspondence should be addressed: Tel: +86-10-64807425; Fax: +86-10-64807429; e-mail: jinc{at}sun.im.ac.cn

Received on October 13, 2008; revised on February 14, 2009; accepted on February 17, 2009

The mammalian ER/cytosolic {alpha}-mannosidase (Man2C1p), yeast vacuolar {alpha}-mannosidase (Ams1p) and the Aspergillus nidulans {alpha}-mannosidase are members of Class IIC subgroup, which is involved in oligosaccharide catabolism and N-glycan processing. Unlike their mammalian counterparts, the yeast Ams1p and A. nidulans Class IIC {alpha}-mannosidase are not essential for morphogenesis and cellular function. In this study, the Afams1, a gene encoding a member of Class IIC {alpha}-mannosidases, was identified in the opportunistic pathogen Aspergillus fumigatus. Deletion of the Afams1 led to a severe defect in conidial formation, especially at a higher temperature. In addition, abnormalities of polarity and septation were associated with the {Delta}Afams1 mutant. Our results showed that the Afams1 gene, in contrast to its homolog in yeast or A. nidulans, was required for morphogenesis and cellular function in A. fumigatus.

Key words: {alpha}-mannosidase / Aspergillus fumigatus / cell wall / morphogenesis / polarity


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