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Glycobiology Advance Access originally published online on October 25, 2008
Glycobiology 2009 19(2):153-159; doi:10.1093/glycob/cwn117
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© The Author 2008. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Complete chemoenzymatic synthesis of the Forssman antigen using novel glycosyltransferases identified in Campylobacter jejuni and Pasteurella multocida

R Scott Houliston2, Stéphane Bernatchez2, Marie-France Karwaski2, Robert E Mandrell3, Harold C Jarrell2, Warren W Wakarchuk2 and Michel Gilbert1,2

2 Institute for Biological Sciences, National Research Council Canada, Ottawa, Ontario, K1A 0R6, Canada
3 US Department of Agriculture, Agricultural Research Service, Produce Safety and Microbiology Research Unit, Albany, CA, USA

1 To whom correspondence should be addressed: Tel: +1-613-991-9956; Fax: +1-613-952-9092; e-mail: michel.gilbert{at}nrc-cnrc.gc.ca

Received on July 1, 2008; revised on October 21, 2008; accepted on October 22, 2008

We have identified an {alpha}1,4-galactosyltransferase (CgtD) and a β1,3-N-acetylgalactosaminyltransferase (CgtE) in the lipooligosaccharide (LOS) locus of Campylobacter jejuni LIO87. Strains that carry these genes may have the capa- bility of synthesizing mimics of the P blood group antigens of the globoseries glycolipids. We have also identified an {alpha}1,3-N-acetylgalactosaminyltransferase (Pm1138) from Pasteurella multocida Pm70, which is involved in the synthesis of an LOS-bound Forssman antigen mimic and represents the only known bacterial glycosyltransferase with this specificity. The genes encoding the three enzymes were cloned and expressed in Escherichia coli as soluble recombinant proteins that can be used to chemoenzymatically synthesize the Forssman antigen, and its biosynthetic precursors, in high yields.

Key words: Campylobacter jejuni / Forssman antigen / glycosyltransferase / Pasteurella multocida


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