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Glycobiology Advance Access originally published online on July 29, 2009
Glycobiology 2009 19(11):1214-1223; doi:10.1093/glycob/cwp112
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© The Author 2009. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

First structural characterization of Burkholderia vietnamiensis lipooligosaccharide from cystic fibrosis-associated lung transplantation strains

Teresa Ieranò2, Alba Silipo2, Luisa Sturiale3, Domenico Garozzo3, Clare Bryant4, Rosa Lanzetta2, Michelangelo Parrilli2, Christine Aldridge5, F Kate Gould6,7, Paul A Corris7,8, C M Anjam Khan5, Anthony De Soyza8 and Antonio Molinaro1,2

2 Dipartimento di Chimica Organica e Biochimica, Università di Napoli Federico II, Napoli, Via Cintia, 4. I-80126
3 CNR-ICTP Catania, Via Gaifami 18, 95126-Catania, Italy
4 Centre for Veterinary Science, Department of Veterinary Medicine, University of Cambridge, Cambridge
5 Institute for Cell and Molecular Biosciences, The Medical School, University of Newcastle
6 Department of Medical Microbiology
7 Cardiopulmonary Transplantation, Freeman Hospital
8 Applied Immunobiology and Transplantation Group, Institute of Cellular Medicine, University of Newcastle, Newcastle, UK

1 To whom correspondence should be addressed: Tel: +39-081-674123; Fax: +39-081-674393; e-mail: molinaro{at}unina.it

Received on June 16, 2009; revised on July 17, 2009; accepted on July 18, 2009

This is the first structural elucidation of the lipooligosaccharide (LOS) endotoxin isolated from Burkholderia vietnamiensis, a clinically important member of Burkholderia cepacia complex, a group of over 10 opportunistic species that are highly problematic in cystic fibrosis. We have characterized a novel LOS structure extracted from two clonal strains of B. vietnamiensis isolated from a cystic fibrosis patient who underwent lung transplantation. Strains were selected from the pretransplantation and post-transplantation periods and endotoxin was extracted. Subsequent analysis interestingly revealed identical oligosaccharidic sequences, but variation in lipid A moieties. Further, both LOS fractions were tested for their immunostimulatory activity on human myelomonocytic U937 cells and for signaling on an HEK293 cell line stably expressing both TLR 4 and MD-2. We observed an increase in lipid A acylation and a resultant increase in biological activity in bio-reporter assays of TNF-{alpha} secretion in the post-transplantation strain.

Key words: Burkholderia vietnamiensis / cystic fibrosis / lipid A / lipopolysaccharide / lung transplantation


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