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Glycobiology Advance Access originally published online on May 5, 2008
Glycobiology 2008 18(7):517-525; doi:10.1093/glycob/cwn034
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© 2008 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Glycation does not modify bovine serum albumin (BSA)-induced reduction of rat aortic relaxation: The response to glycated and nonglycated BSA is lost in metabolic syndrome

Maria Esther Rubio-Ruiz2, Eulises Díaz-Díaz4, Mario Cárdenas-León4, Rabindranath Argüelles-Medina4, Patricia Sánchez-Canales4, Fernando Larrea-Gallo4, Elizabeth Soria-Castro3 and Verónica Guarner-Lans1,2

2 Department of Physiology
3 Department of Pathology, Instituto Nacional de Cardiología "Ignacio Chávez"
4 Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y de la Nutrición "Salvador Zubirán," Mexico City, Mexico

1 To whom correspondence should be addressed: Tel: +52-55-55-73-29-11, Ext: 1222; Fax: +52-55-55-73-09-26; e-mail: gualanv{at}yahoo.com

Received on September 26, 2007; revised on April 11, 2008; accepted on April 25, 2008

The effects of nonglycated bovine serum albumin (BSA) and advanced glycosylation end products of BSA (AGE-BSA) on vascular responses of control and metabolic syndrome (MS) rats characterized by hypertriglyceridemia, hypertension, hyperinsulinemia, and insulin resistance were studied. Albumin and in vitro prepared AGE-BSA have vascular effects; however, recent studies indicate that some effects of in vitro prepared AGEs are due to the conditions in which they were generated. We produced AGEs by incubating glucose with BSA for 60 days under sterile conditions in darkness and at 37°C. To develop MS rats, male Wistar animals were given 30% sucrose in drinking water since weanling. Six month old animals were used. Blood pressure, insulin, triglycerides, and serum albumin were increased in MS rats. Contraction of aortic rings elicited with norepinephrine was stronger. There were no effects of nonglycated BSA or AGE-BSA on contractions in control or MS rats; however, both groups responded to L-NAME, an inhibitor of nitric oxide synthesis. Arterial relaxation induced using acetylcholine was smaller in MS rats. Nonglycated BSA and AGE-BSA significantly diminished relaxation in a 35% in the control group but the decrease was similar when using nonglycated BSA and AGE-BSA. This decrease was not present in the MS rats and was not due to increased RAGEs or altered biochemical characteristics of BSA. In conclusion, both BSA and AGE-BSA inhibit vascular relaxation in control artic rings. In MS rats the effect is lost possibly due to alterations in endothelial cells that are a consequence of the illness.

Key words: AGE-BSA / BSA / metabolic syndrome / vascular relaxation


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