Skip Navigation


Glycobiology Advance Access originally published online on January 3, 2008
Glycobiology 2008 18(3):250-259; doi:10.1093/glycob/cwm139
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
18/3/250    most recent
cwm139v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Farias, E. H C
Right arrow Articles by Mourão, P. A S
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Farias, E. H C
Right arrow Articles by Mourão, P. A S
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum

Eduardo H C Farias2,3, Vitor H Pomin2,4,5, Ana-Paula Valente5,6, Helena B Nader7, Hugo A O Rocha1,3 and Paulo A S Mourão1,4,5

3 Laboratório de Biotecnologia de Polímeros Naturais - BIOPOL, Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, RN, 59072-970
4 Laboratório de Tecido Conjuntivo, Hospital Universitário Clementino Fraga Filho
5 Instituto de Bioquímica Médica, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Caixa Postal 68041, Rio de Janeiro, RJ, 21941-590
6 Centro Nacional de Ressonância Magnética Nuclear Jiri Jonas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-590
7 Departamento de Bioquímica, Universidade Federal de São Paulo, SP, 04044-020, Brazil

1 To whom correspondence should be addressed: e-mail: pmourao{at}hucff.ufrj.br

Received on October 9, 2007; revised on November 28, 2007; accepted on December 28, 2007

The green algae of the genus Codium have recently been demonstrated to be an important source of sulfated galactans from the marine environment. Here, a sulfated galactan was isolated from the species Codium isthmocladum and its structure was studied by a combination of chemical analyses and NMR spectroscopy. Two fractions (SG 1, ~14 kDa, and SG 2, ~20 kDa) were derived from this highly polydisperse and heterogeneous polysaccharide. Both exhibited similar structures in 1H 1D NMR spectra. The structural features of SG 2 and its desulfated derivative were analyzed by COSY, TOCSY, DEPT-HSQC, HSQC, and HMBC. This sulfated galactan is composed preponderantly of 4-sulfated, 3-linked β-D-galactopyranosyl units. In minor amounts, it is sulfated and glycosylated at C-6. Pyruvate groups are also found, forming five-membered cyclic ketals as 3,4-O-(1'carboxy)-ethylidene-β-D-galactose residues. A comparison of sulfated galactans from different marine taxonomic groups revealed similar backbones of 3-β-D-Galp-1.

Key words: Codium isthmocladum / green alga / green seaweed / phylogeny / pyruvylated-sulfated galactan

2 These two authors contributed equally to the work.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. O. Castro, V. H. Pomin, L. L. Santos, A.-C. E. S. Vilela-Silva, N. Hirohashi, L. Pol-Fachin, H. Verli, and P. A. S. Mourao
A Unique 2-Sulfated {beta}-Galactan from the Egg Jelly of the Sea Urchin Glyptocidaris crenularis: CONFORMATION FLEXIBILITY VERSUS INDUCTION OF THE SPERM ACROSOME REACTION
J. Biol. Chem., July 10, 2009; 284(28): 18790 - 18800.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
J. M. Estevez, P. V. Fernandez, L. Kasulin, P. Dupree, and M. Ciancia
Chemical and in situ characterization of macromolecular components of the cell walls from the green seaweed Codium fragile
Glycobiology, March 1, 2009; 19(3): 212 - 228.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
V. H Pomin and P. A S Mourao
Structure, biology, evolution, and medical importance of sulfated fucans and galactans
Glycobiology, December 1, 2008; 18(12): 1016 - 1027.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.