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Glycobiology Advance Access originally published online on December 3, 2007
Glycobiology 2008 18(2):158-165; doi:10.1093/glycob/cwm129
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© The Author 2008. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Communication

Sensitive detection of isoglobo and globo series tetraglycosylceramides in human thymus by ion trap mass spectrometry

Yunsen Li2, Susann Teneberg3, Prakash Thapa2, Albert Bendelac4, Steven B. Levery1,5 and Dapeng Zhou1,2

2 Department of Melanoma Medical Oncology, The University of Texas M.D. Anderson Cancer Center, Houston, TX 77054, USA
3 Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology, Göteborg University, SE-40530 Göteborg, Sweden
4 Howard Hughes Medical Institute, Department of Pathology and Committee of Immunology, University of Chicago, Chicago, IL 60637, USA
5 Department of Chemistry, University of New Hampshire, Durham, NH 03824-3598, USA

1 To whom correspondence should be addressed: Tel: +1-713-792-3134; Fax: +1-713-563-3424; e-mail: dzhou{at}mdanderson.org and Tel: +1-603-862-2529; Fax: +1-603-862-4278; e-mail: slevery{at}cisunix.unh.edu

Received on September 30, 2007; revised on November 25, 2007; accepted on November 28, 2007

Glycosphingolipids serve as ligands for receptors involved in signal transduction and immune recognition, as exemplified by isoglobotrihexosylceramide, an antigenic ligand for T cell receptors. Mechanistic studies on the regulation of isoglobotrihexosylceramide require biochemical measurement of its lysosomal precursor, isoglobotetraglycosylceramide. It remains a challenge to distinguish between complex tetraglycosylceramide glycosphingolipid isomers with the same sugar components but diverse internal linkages. Here we established a simple and sensitive method to separate globo- and isoglobotetraglycosylceramide by MS5 ion trap mass spectrometry, and report the identification of isoglobotetraglycosylceramide in a CHO cell line transfected by iGb3 synthase, as well as in human thymus.

Key words: CD1d / glycosphingolipid / ion trap MS / isoglobotetraglycosylceramide / natural killer T cells


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