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Glycobiology Advance Access originally published online on December 3, 2007
Glycobiology 2008 18(2):145-151; doi:10.1093/glycob/cwm128
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© The Author 2008. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Communication

Neural complex-specific expression of xylosyl N-glycan in Ciona intestinalis

Hirokazu Yagi2,3,, Masashi Nakagawa4, Noriko Takahashi2,3,5,6,, Sachiko Kondo2,5,, Mamoru Matsubara7 and Koichi Kato1,2,3,5,6,8,

2 Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603, Japan
3 CREST, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi 332–0012, Japan
4 Graduate School of Life Science, University of Hyogo, 3-2-1 Koto, Akoh-Gun. Hyogo 678-1297, Japan
5 GLYENCE Co., Ltd., 2-22-8 Chikusa, Chikusa-ku, Nagoya 464-0858, Japan
6 The Glycoscience Institute, Ochanomizu University, 2-1-1 Ohtsuka, Bunkyo-ku, Tokyo 112-8610, Japan
7 Faculty of Bioenvironmental Science, Kyoto Gakuen University, 1-1 Nanjyo-ohtani, Kameoka 621-8555, Japan
8 Institute for Molecular Science, National Institutes of Natural Sciences, 5-1 Higashiyama Myodaiji, Okazaki 444-8787, Japan

1 To whom correspondence should be addressed: Tel: +81-52-836-3447; Fax: +81-52-836-3447; e-mail: kkato{at}phar.nagoya-cu.ac.jp

Received on September 22, 2007; revised on November 25, 2007; accepted on November 26, 2007

We herein report N-glycosylation profiles of the individual tissues derived from the ascidian Ciona intestinalis. Multidimensional HPLC mapping revealed that the C. intestinalis expresses high-mannose-type oligosaccharides as major N-glycans, along with paucimannose-type and complex-type oligosaccharides, in a tissue-specific manner. Notably, the trimannosyl core carrying β1,2-xylose and {alpha}1,3-fucose residues was identified as a principal N-glycan in the neural complex. As far as we know, this is the first description of xylosyl N-glycan expressed in deuterostome. Furthermore, we found that this xylosyl N-glycan is exclusively displayed on a membrane-associated protein so far described as a putative protein whose gene expression is specific for the neural complex. These data suggested that the xylosyl N-glycan is associated with some neural functions of C. intestinalis.

Key words: ascidian / Ciona intestinalis / neural complex / N-glycan / xylose


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